Role of BAP in In-vitro Conditions for Promoting Shoot Growth in the Nodal Segment of Rosa indica L.
Uma Sharma *
College of Biotechnology, Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura-281001, India.
Neha Pathak
Department of Botany, Sanskriti University, Mathura-281001, India.
*Author to whom correspondence should be addressed.
Abstract
Rosa indica L. is the world's most popular ornamental flowering plant. Since the last two decades, rose tissue culture has been enhanced and used for a variety of objectives, ranging from fundamental anatomical and physiological studies to micropropagation from auxiliary buds, shoot tips, leaf explants, and so on. In in vitro conditions, surface sterilized nodal segments of Rosa indica L. were cultivated in MS media supplemented with 30 g/l sugar as a carbon source, 6 g/l agar as a solidifying agent, and various concentrations of BAP (1, 2 and 3 mg/l). Maximum growth of shoots in nodal segments of Rosa indica L. was observed on MS medium having 2.0 mg/l of BAP concentration as compared to other concentrations. The results showed that BAP was considered to be the best phytohormone for in vitro shoot multiplication and proliferation of Rosa indica L.
Keywords: Rosa indica L, microprogation, nodal segment, BAP