Detection of Antibiotic Resistance Genes bla SHV, bla TOHO and bla NDM in Pathogenic Gram-Negative Bacilli at the Pietro Annigoni Biomolecular Research Center
Rhaina Olivia Badini
Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR-SVT, Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso and Faculty of Medicine, Saint Thomas Aquinas University (USTA), 06 BP 10212 Ouagadougou 06, Burkina Faso.
Amana Mètuor Dabiré
Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR-SVT, Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso and University of Dédougou, BP 176 Dédougou, Dédougou, Burkina Faso.
Abdoul Karim Ouattara *
Norbert Zongo University, Manga University Center, BP 376 Koudougou, Burkina Faso.
Rahimatou Yasmine Wend-Kuni Tiemtoré
Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR-SVT, Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Serge Sougué
Laboratoire National de Santé Publique de Ouagadougou, Ouagadougou, Burkina Faso.
Théodora Mahoukèdè Zohoncon
Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR-SVT, Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso and Faculty of Medicine, Saint Thomas Aquinas University (USTA), 06 BP 10212 Ouagadougou 06, Burkina Faso.
Jacques Simporé
Laboratory of Molecular Biology and Genetics (LABIOGENE), UFR-SVT, Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso and Faculty of Medicine, Saint Thomas Aquinas University (USTA), 06 BP 10212 Ouagadougou 06, Burkina Faso and Pietro Annigoni Biomolecular Research Center (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Gram-negative bacilli, notably Enterobacteriaceae, are increasingly resistant to antibiotics thanks to the acquisition and dissemination of extended-spectrum β-lactamases (ESBLs). The present study aims to identify the bla NDM, bla SHV and bla TOHO genes in clinical strains of Gram-negative bacteria isolated from patients at the Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou. The isolation and purification of bacterial strains isolated from the stools and urine of internal and external CERBA patients were carried out respectively on selective and Muller Hinton (MH) media. The antibiogram was performed using the disk diffusion method. The API 20E biochemical gallery (BioMérieux, France) was used for the identification of Enterobacteriaceae while the bla NDM, bla SHV and bla TOHO genes were detected by conventional PCR. A total of thirty-seven (37) strains of Gram-negative bacilli were included in the present study. The antibiogram showed that 62.16% (23/37) of them were ESBL producers with 56.52% (13/23) of Escherichia coli; 39.13% (9/23) of Klebsiella sp. and 4.35% (1/23) of Proteus sp. Among them, 60.87% (14/23) of the strains harbored the bla NDM gene, 56.52% (13/23) bla SHV against 47.83% (11/23) of the strains carrying the bla TOHO gene. The combinations of bla SHV + bla NDM, bla SHV + bla TOHO and bla TOHO + bla NDM genes were found in 30.43% (7/23), 26.08 (6/23) and 17.39% (4 /23) strains, respectively against 8.70% (2/23) of strains carrying bla SHV + bla NDM + bla TOHO. It should also be noted that most of these strains were isolated in urine cultures. This study revealed clinical strains of ESBL-producing Enterobacteriaceae carrying the bla NDM, bla SHV and bla TOHO genes. The simultaneous carriage of two or three genes by certain strains suggests dissemination which requires increased surveillance efforts and the rapid development of new therapeutic solutions.
Keywords: ESBL, antibiotic resistance, enterobacteria, blaSHV, blaTOHO, blaNDM