Selection of Powdery Mildew Resistant Brassica Genotypes Based on Disease Indexing and Microsatellite Markers
Aditi Shrivastava
Department of Genetics and Plant Breeding, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior -474002, India.
Manoj Kumar Tripathi *
Department of Genetics and Plant Breeding, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior -474002, India and Department of Plant Molecular Biology and Biotechnology, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior -472004, India.
Sushma Tiwari
Department of Genetics and Plant Breeding, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior -474002, India and Department of Plant Molecular Biology and Biotechnology, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior -472004, India.
Niraj Tripathi
Directorate of Research, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur-482004, India.
Prakash N. Tiwari
Department of Plant Molecular Biology and Biotechnology, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior -472004, India.
Purnima Singh
Department of Plant Pathology, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior-472004, India.
Prerana Parihar
Department of Plant Pathology, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior-472004, India.
Rakesh Yadav
Department of Genetics and Plant Breeding, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior -474002, India.
Shailja Chauhan
Department of Genetics and Plant Breeding, College of Agriculture, Rajmata Vijayaraje Scindia Agricultural University, Gwalior -474002, India.
J. Singh
Zonal Agricultural Research Station, Morena, Rajmata Vijayaraje Scindia Agricultural University, Gwalior 474002, India.
*Author to whom correspondence should be addressed.
Abstract
Powdery mildew disease of oilseed mustard caused by Erysiphe cruciferarum is a primary reason of yield reduction not only in India but also throughout the world. Identification and cultivation of resistant mustard genotypes against powdery mildew is the only way to overcome this challenge. In the present investigation, we targeted to screen 75 Brassica genotypes against powdery mildew based on disease indexing under field conditions and gene-specific molecular markers. Disease reaction on both the cotyledonary and true leaves was screened with using a modified 0-9 scale score as well as with nineteen disease linked microsatellite markers. In disease indexing under field conditions, genotypes viz., L-4 and PC-5 were identified as immune, China and RP-9 were considered as highly resistant and GSC-7 and PC-6 were recognized as resistant whilst genotypes i.e., RB-50, Pusa Bold, WRR-10 and GSL-1 were accredited as moderately resistant. Molecular markers based UPGMA dendrogram classified Rohini, WRR-22, PC-6, PusaBold, China, WRR-8, GSL-1, WRR-7, RH-749, L-4 and RB-50 as highly resistant mustard genotypes. In addition, disease linked marker cnu_m616 had the highest polymorphic information content (0.75) with greatest ability to differentiate resistant genotypes from susceptible genotypes, may be employed directly in mustard breeding programmes in future.
Keywords: Mustard, powdery mildew, disease indexing, gene-linked markers, pathogens