Pharmacological Repositioning of Thiocolchicoside: Antibacterial Evaluation In vitro
Dicla Aline Semedo da Veiga
Academic Unit of Life Sciences (UACV), Teacher Training Center (CFP), Federal University of Campina Grande (UFCG), Cajazeiras, Paraíba-58900-000, Brazil.
Hélida Maravilha Dantas e Sousa Almeida
Postgraduate Program in Pharmaceutical Sciences, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, University Campus, Lagoa Nova, Natal - RN, 59078-970, Brazil.
Emmanuel Florêncio Leite
Cajazeiras Health Technical School, Cajazeiras, Paraíba-58900-000, Brazil.
Kevyn Gabriel Mascarenha
Cajazeiras Health Technical School, Cajazeiras, Paraíba-58900-000, Brazil.
Lívia Maria das Chagas Rocha
Cajazeiras Health Technical School, Cajazeiras, Paraíba-58900-000, Brazil.
Sara Lohanna Saraiva França
Cajazeiras Health Technical School, Cajazeiras, Paraíba-58900-000, Brazil.
Sávio Benvindo Ferreira *
Academic Unit of Life Sciences (UACV), Teacher Training Center (CFP), Federal University of Campina Grande (UFCG), Cajazeiras, Paraíba-58900-000, Brazil.
*Author to whom correspondence should be addressed.
Abstract
Pharmacological repositioning has been increasingly praised as a viable, low-cost and rapid alternative for the development of a new therapy for clinical application, such as cases of bacterial resistance. Therefore, the present study aimed to investigate the antibacterial action of the myorelaxant thiocolchicoside. For this, an in vitro experimental study was developed using the bacterial strains Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Proteus mirabilis ATCC 25933 and Pseudomonas aeruginosa ATCC 27853, and the protocols: antibacterial activity screening, Minimum Inhibitory Concentration (MIC) and characterization of antibacterial activity. The substance was thiocolchicoside, in concentrations ranging from 0.48 to 1000 µg/mL. Low sensitivity of bacterial strains to the myorelaxant was observed, obtaining an MIC of 500 µg/mL for E. coli and 1000 µg/mL for P. aeruginosa, the only strains sensitive to the compound. In the antibacterial activity characterization test, thiocolchicoside showed bacteriostatic action. The conclusions this research indicate the need to studies are needed on its action in comparison with other microorganisms, such as other bacterial species, fungi and protozoa, in order to evaluate its effect on other pathogenic organisms.
Keywords: Antibacterial activity, bacterial resistance, pharmacological repositioning, thiocolchicoside