Rapid Method for Genomic DNA Isolation of Mungbean [Vignar adiata (L.) Wilczek]
Sampat Kumar Kumawat
Department of Agricultural Biotechnology and Molecular Biology, CBS&H, Pusa, India.
Bishun Deo Parsad *
Department of Agricultural Biotechnology and Molecular Biology, CBS&H, Pusa, India.
Sangita Sahni
Department of Plant Pathology, TCA, Dholi Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur-848125, Bihar, India.
*Author to whom correspondence should be addressed.
Abstract
The most significant pulse crop in India is the mungbean [Vignar adiata (L.) Wilczek], which constitutes almost 16% of the national pulse area. For biotechnological interventions, it is prerequisite to develop a rapid and cost-effective method for genomic DNA isolation suitable for PCR. In present investigation,modified CTAB method was used to extract the genomic DNA from the leaf tissues of mungbesn. By crushing the leaves of several mungbean genotypes in a pre-heated CTAB extraction buffer, genomic DNA was extracted. Further, SSR markers were used to assess the efficiency of the extracted DNA.The SSR marker-based PCR amplification findings showed that the DNA extracted using this approach was of good quality and suitable for SSR analysis.
Keywords: Mungbean [Vignar adiata (L.) Wilczek], genomic DNA, Cetyl Trimethyl Ammonium Bromide (CTAB), SSR marker, PCR amplification