Detection of Heterozygous o2O2 Allele in BC1F1 Progeny of QPM Versus Normal Cross in Maize Using Marker-Assisted Selection
Shamshir Ul Hussan *
Division of Genetics and Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
M. Ashraf Rather
Division of Genetics and Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
Zahoor A. Dar
Dryland Agriculture Research Station, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
Asif B. Shikari
Division of Genetics and Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
M. Altaf Wani
Division of Genetics and Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
Zaffer Mahdi Dar
Division of Basic Sciences, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
M. Anwar Bhat
Division of Agronomy, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
Fehim Jeelani Wani
Division of Agricultural Statistics and Economics, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
Rukhsana Jan
Division of Agronomy, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
Aflaq Hamid
Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
Shahjahan Rashid
Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
Azra Khan
Division of Genetics and Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
Nusrat Ul Islam
Division of Genetics and Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
Mohsin Ahmad Hajam
Division of Fruit Science, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
M. Aasif Sheikh
Division of Entomology, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), UT of J&K, India.
*Author to whom correspondence should be addressed.
Abstract
Marker assisted back crossing is reliable tool in selecting the heterozygotes in backcross generations. It quickens the selection process which would otherwise require an extra generation/season for raising the BCF2 seed for finding the recessive allele which needs to be phenotypically expressed. Therefore in the present study normal maize (BML-6and IML-187) and QPM donor lines (DQL-2029-1 and DQL-779-2-9) were genotyped at o2 locus using co-dominant SSR marker phi057. The marker allele size at 02 locus for normal parents was 155 bp in IML-187(P1) and 160 bp in BML-6(P2). 170bp in DQL-2029-1(P3) and DQL-779-2-9(P4) for QPM or donor parents. Based on this polymorphism crosses were made to generate F1 hybrids. IML-87 was crossed with DQL-2029-1 and BML-6 was crossed with DQL-779-2-9. Thus, based on polymorphism at the DNA level, biparental backcrosses were made to eventually combine o2 allele in the endosperm of normal maize. The two BC1F1 populations were genotyped at the opaque-2 locus using phi057 SSR marker. It was observed that out of 96 plants analyzed, 40 individuals were heterozygous at o2 locus in BC1F1 population derived from the cross BC1F1 (A) while 43 out of the 100 plant analyzed in cross BC1F1 (B) were heterozygous when screened with phi057 SSR marker. Marker Assisted backcrossing is a useful tool in trait introgression as it helps in pre-flowering elimination of negative plants as well as donor genome and need for selfing is eliminated in BCF1 s. SSR phi-057 was able to clearly distinguish between QPM and normal parents and detect heterozygous plants in the backcross progeny.
Keywords: SSR, marker assisted selection, marker assisted backcrossing, heterozygote, maize