Screening for Bacterial Leaf Blight Resistant Genes in Some Rice Genotypes Using SSR Markers
Sayani Bandyopadhyay *
Department of Agricultural Biotechnology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia -741252, West Bengal, India.
Tapash Dasgupta
School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata-700103, West Bengal, India.
Samita Saha
School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata-700103, West Bengal, India.
Nillahit Payra
School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda Educational and Research Institute, Narendrapur, Kolkata-700103, West Bengal, India.
*Author to whom correspondence should be addressed.
Abstract
Bacterial leaf blight disease plays a major detrimental effect in the yield and quality of Rice (Oryza sativa). To overcome the problem, finding bacterial leaf blight resistant gene is very crucial. In this study, identification of bacterial leaf blight resistant genes was attempted among 27 rice genotypes including two control genotypes using three Simple Sequence Repeats markers tagged with Bacterial leaf blight resistant genes which are Xa4, xa5 and xa13. As a result, six genotypes, MTU1010, IR 68144-2B-2-2-3-1-127, ARC-10086, Mali 4, IR 64 and Kalinga-2, were classified as resistant to the disease due to the presence of bands (150bp, 160bp and 190bp), of MP1, RM13 and RM264 markers which were tagged with Xa4, xa5 and xa13 genes respectively. Among the genotypes, 12 genotypes were considered as susceptible and the other 7 genotypes showed moderate resistance or susceptible results. The identified resistant genotypes can be utilized as a donor for developing Bacterial leaf blight resistant rice varieties in breeding programme.
Keywords: Bacterial leaf blight, marker, rice, resistant, simple sequence repeats