Antinutritional and Protein Based Profiling of Diverse Desi and Wild Chickpea Accessions
Neha Gupta
School of Studies in Biotechnology, Jiwaji University, Gwalior-474011, Madhya Pradesh, India and Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior- 474002, Madhya Pradesh, India.
Sushma Tiwari
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior- 474002, Madhya Pradesh, India.
M. K. Tripathi
School of Studies in Biotechnology, Jiwaji University, Gwalior-474011, Madhya Pradesh, India.
Sameer S. Bhagyawant
*
School of Studies in Biotechnology, Jiwaji University, Gwalior-474011, Madhya Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Background: To design future breeding programs, biochemical analysis is fundamental. Chickpea is a major protein source in India and worldwide. More than 3000 chickpea seed accessions are being maintained by Indian Institute of Pulses Research (IIPR), Kanpur which demand biochemical analysis.
Methods: Present study pertains multivariate analysis based on antinutritional content and their protein profile of 20 accessions of Cicer that included cultivated desi and wild.
Results: The spectrum of biochemical characteristics was documented; for lectin ranged 192.19 HU/mg to 12.26 HU/mg and total proteins varied from 2.66-0.59 mg/g. SDS-PAGE appraised various bands in a molecular weight range of 3.5 to 125 kDa acknowledging genetic diversity. On the basis of present study, accession selection for future breeding programs to develop nutritionally elite chickpea cultivar can be executed.
Keywords: Wild chickpea, Lectin, SDS-PAGE, total methionine