A Rapid and Inexpensive Method of DNA Extraction from Palmyra Palm (Borassus flabellifer)
Soni Kumari
Department of Horticulture (Fruit & Fruit Technology), Bihar Agricultural University (B.A.U.), Sabour, Bhagalpur-813210, Bihar, India.
Ruby Rani *
Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University (B.A.U.), Sabour, Bhagalpur-813210, Bihar, India.
Jitesh Kumar
Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University (B.A.U.), Sabour, Bhagalpur-813210, Bihar, India.
Ravi Ranjan Kumar
Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University (B.A.U.), Sabour, Bhagalpur-813210, Bihar, India.
Tushar Ranjan
Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University (B.A.U.), Sabour, Bhagalpur-813210, Bihar, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: The study aims to highlight the simple optimisation, inexpensive and rapid procedure for DNA isolation from tough leaves (Palmyra palm) without compromising the yield and purity of DNA.
Study Design: Leaf of palmyra palm (Borassus flabellifer) was used to conduct the experiment followed by laboratory analysis, DNA extraction and PCR amplification.
Results and Discussion: The results showed that different buffers examined for the extraction of DNA provided significantly different levels of yield and purity. DNA isolated by lysis buffers C showed satisfactory amplifications in PCR. The fingerprint we obtained by using the DNA extracted by these buffers provided higher resolution than those using buffers.
Conclusion: This study suggests that grinding of Palmyra palm leaves with sterile sand or cover slips and inclusion of SDS, Tween 20, and NaCl (1.4 M) in the lysis buffer without the costly use of liquid nitrogen, PVP and β mercaptoethanol, provides a DNA yield of sufficient purity, suitable for PCR amplification and subsequent use.
Keywords: Palmyra, DNA, extraction, amplification, quality