Assessment of Genetic Diversity in Hybrid Litchi (Litchi chinensis Sonn.) Revealed by RAPD Marker
Abha Kumari
Department of Horticulture (Fruit and Fruit Technology), Bihar Agricultural University, Sabour, Bhagalpur-813210, Bihar, India.
Bishun Deo Prasad *
Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur-813210, Bihar, India.
Pankaj Kumar
Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur-813210, Bihar, India.
Ruby Rani
Department of Horticulture (Fruit and Fruit Technology), Bihar Agricultural University, Sabour, Bhagalpur-813210, Bihar, India.
*Author to whom correspondence should be addressed.
Abstract
The genetic diversity in 10 selected hybrids of litchi, Litchi chinensis Sonn. using 15 decamer random primers. Out of 15, 10 RAPD primers revealed polymorphism while the remaining 5 primers reflected no polymorphism. The primers produced a total of 52 bands of which 33 were polymorphic (61.11%). The number of polymorphic fragments for each primer varied from 1-7 with an average of 1.84 polymorphic fragments. The RAPD data were analysed to determine the genetic similarity coefficients which ranged from 0.57 to 0.83. Cluster analysis was performed using the Jaccard's similarity coefficient. This method dendrogram resolved the selected litchi hybrids into two major clusters. The results reveal the genetic relationship of hybrids of litchi developed at BAU, Sabour- Bhagalpur (Bihar).
Keywords: Litchi hybrids, genetic variation, RAPD, genetic similarities, PCA.