Molecular Detection and Diversity Analysis of Colletotrichum sp from Chilli Using RAPD-PCR
V. Karthik Pandi *
Department of Plant Pathology, Centre for plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India.
A. Kamalakannan
Department of Plant Pathology, Centre for plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India.
S. Nakkeeran
Department of Plant Pathology, Centre for plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India.
K. Venkatesan
Coconut Research Station, Aliyarnagar-642 101, Tamil Nadu, India.
D. Uma
Department of Biochemistry, Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
The molecular variability among the isolates of Colletotrichum capsici and Colletotrichum gloeosporioides that differed in virulence was analysed by means of random amplified polymorphic DNA (RAPD) using 10 random primers. Analysis of the genetic coefficient matrix derived from the scores of RAPD profile showed that maximum per cent similarities between species of C. capsici and C. gloeosporioides which were in the range of 92% respectively. Cluster analysis using the unweighted pair-group method with arithmetic average (UPGMA) clearly separated the isolates into two clusters (I and II), confirming the genetic diversity among the isolates of C. capsici and C. gloeosporioides from chilli. RAPD analysis in the present study showed a clear difference between C. gloeosporioides and C. capsici. Furthermore, C. capsici isolates were found to be more closely related than C. gloeosporioides isolates.
Keywords: C. capsici, C. gloeosporioides, molecular variability