Phytochemical Screening, Evaluation of Antioxidant and Anti-sickling Activities of Two Polar Extracts of Combretum glutinosum Leaves. Perr. ex DC
Cheikh Sall *
Laboratory of Chemistry, Training and Research Unit of Health, Thies University, PB 967 Thies, Sénégal.
Samba Fama Ndoye
Laboratory of Organic and Therapeutic Chemistry, Faculty of Medicine, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), PB 5005 Dakar-Fann, Sénégal
Mbaye Diaw Dioum
Laboratory of Organic and Therapeutic Chemistry, Faculty of Medicine, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), PB 5005 Dakar-Fann, Sénégal
Insa Seck
Laboratory of Organic and Therapeutic Chemistry, Faculty of Medicine, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), PB 5005 Dakar-Fann, Sénégal
Rokhaya Sylla Gueye
Laboratory of Organic and Therapeutic Chemistry, Faculty of Medicine, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), PB 5005 Dakar-Fann, Sénégal
Babacar Faye
Laboratory of Immunology, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), Sénégal
Cheikhou Omar Thiam
Laboratory of Organic and Therapeutic Chemistry, Faculty of Medicine, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), PB 5005 Dakar-Fann, Sénégal
Matar Seck
Laboratory of Organic and Therapeutic Chemistry, Faculty of Medicine, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), PB 5005 Dakar-Fann, Sénégal
Papa Madièye Gueye
Laboratory of Pharmaceutical Biochemistry, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), Sénégal
Djibril Fall
Laboratory of Organic and Therapeutic Chemistry, Faculty of Medicine, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), PB 5005 Dakar-Fann, Sénégal
Mamadou Fall
Laboratory of Toxicology and Hydrology, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), Sénégal
Tandakha Ndiaye Dieye
Laboratory of Immunology, Pharmacy and Odontology (F.M.P.O.), Cheikh Anta Diop University (U.C.A.D.), Sénégal
*Author to whom correspondence should be addressed.
Abstract
Sickle cell disease is a major public health problem in Africa and many other areas across the world. In Senegal, a lot of plants are proposed by traditional healers to manage the sickle cell disease, among them Combretum glutinosum. Antisickling activity of Combretum glutinosum leaves extracts was studied in this work. Methanolic and ethyl acetate extracts of Combretum glutinosum leaves were evaluated on SS sickles type to determine their anti-sickling potential. Antiradical properties of methanolic extract were evaluated using the DPPH radical as oxidant. Total phenolic content of the methanol extract was determined. Phytochemical screening of the crude extract of methanol revealed the presence of tannins, saponins, phenols and flavonoids. Results show a good antisickling effect of extracts with a maximum antisickling revers of 81% and 69% respectively, of the methanol and ethyl acetate extracts at 10 mg/mL versus 67% for arginine at 120 min of incubation. The measured IC50 were 0.65 and 0.163 for respectively the methanol extract and ascorbic acid. Antiradical powers 0.155 and 0.62 respectively for methanol extract and ascorbic acid were calculated from the effective concentrations. The results of this study confirm the traditional use of Combretum glutinosum leaves in the management of sickle cell disease.
Keywords: Sickle cell disease, oxidative stress, medicinal plant, Combretum glutinosum, antisickling activity, antioxidant activity