Open Access Short Communication

First Report of Diplodia mutila on Blue Pine (Pinus wallichiana) in Kashmir Valley, India

Shubana Bhat, M. A. Beig, G. H. Dar, Seemi lohani Purshottam Singh, Farahanaz Rasool, Parveez Ahmad Sheikh, Waseem Ali Dar Ahmed Dar, Hilal Ahmad Bhat, Sushil Kumar

Current Journal of Applied Science and Technology, Page 1-2
DOI: 10.9734/cjast/2021/v40i2631518

Blue pine (Pinus wallichiana) is prone to a number of diseases amongst which needle blight caused by Diplodia mutilaposes a serious threat to its regeneration in Kashmir valley. The disease initiated in the month of March as minute chlorotic spots on current year needles.

Open Access Original Research Article

Degradation of Pyrazosulfuron Ethyl in Rice Ecosystem by Aspergillus terreus

Shiv Shankar Mahesh, P. P. Choudhary, Santi Ranjan Mitro, Amit Raj

Current Journal of Applied Science and Technology, Page 3-10
DOI: 10.9734/cjast/2021/v40i2631519

Investigation on the degradation of pyrazosulfuron ethyl by Aspergillus terreus in the rice ecosystem was conducted at the Department of Biochemistry, UBKV, Pundibari Coochbehar, West Bengal, India. The soil was collected from the paddy field to isolate fungal inoculums. Results revealed that Aspergillus terreus can survive in minimal broth containing pyrazosulfuron ethyl  at 1000 ppm and degrades through two major pathways, first involves the cleaves of sulfonylurea bridge resulting in the formation of two major metabolites viz., 2-amino-4, 6 –dimethoxyprimidine, 5-aminosulfonyl-1-methyl-pyrazole-4-carboxylic ethyl ester and, second was the cleavage of sulfonyl amide linkage which forms the metabolite viz; 1 methyl pyrazole-4-carboxylic acid ethyl ester,1 methyl pyrazole -4-carboxylic acid, 5-carbamoyl -1-methyl pyrazole -4-carboxylic acid ethyl-ester. The enzyme involved in these transformations can be utilized to decontaminate soil and water from Pyrazosulfuron ethyl residue. Even, the gene responsible for the production of these useful enzymes can be exploited for future research.

Open Access Original Research Article

Allelopathic Effects of Extract Robinia pseudoacacia L. and Chenopodium album L. on Germination of Tomato (Solanum lycopersicum L.)

Sanida Bektić, Samira Huseinović, Jasminka Husanović, Senad Memić

Current Journal of Applied Science and Technology, Page 11-18
DOI: 10.9734/cjast/2021/v40i2631520

Allelopathy can have an important applicaiton in areas of agriculture, especially in integrated protection from weeds, by using of allelopathic crops in different ways. In this research allelopathic effects of invasive species acacia (Robinia pseudoacacia L.)  and white goosefood (Chenopodium album L.) are explored on germination of   tomato (Solanum lycopersicum L.). Water extracts of dry leaves of white goosefoot and acacia are prepared according tothe  method : Norsworthy (2003). Experiment has been made in controlled laboratory conditions. Results of this research show that acacia and white goosefood have negative allelopathy potential and they act inhibitory on germination of tomatoes. Research of allelopathy and allelopathic relationships of weed species and agricultural cultures represents a big challenge for those people who are working in food production, and at the same time can be an instrument of ecologically sustainable agriculture.

Open Access Original Research Article

Loop Mediated Isothermal Amplification as a Rapid Molecular Method for Pseudomonas aeruginosa T3SS ExoY Gene Septicemia Detection in Beta- Lactamase Species Co-Infections

J. Mageto Ombega, Zhao-Hua Zhong

Current Journal of Applied Science and Technology, Page 19-29
DOI: 10.9734/cjast/2021/v40i2631521

Background: Pseudomonas aeruginosa is among the most important causative agent of infection in chronically ill patients admitted in hospitals globally. Coupled with its, mixed symptomatology, rapid drug resistance tendency and its causation of severe disease, a fast, reliable and affordable diagnostic technique is required to enable healthcare providers expeditiously mitigate its progression and eventual treatment. The Loop-Mediated Isothermal Amplification (LAMP) technique has the potential to serve as a simple, rapid, specific, sensitive and cost-effective point-of-care diagnostic tool.

Broad Objective: To investigate Loop Mediated Isothermal Amplification as a molecular technique for microbial diagnostic and prognostic predictor.  

Study Design: This study was aimed at evaluating LAMP assay against Simple Polymerase chain reaction and Multiplex PCR on the diagnosis of P. aeruginosa in mixed clinical samples.

Materials and Methods: This study developed P. aeruginosa Loop Mediated Isothermal Amplification (PaLAMP) assay to target the ExoY gene with appropriate primer testing and validation procedures. Culture of patient bacterial samples was done on MHA and MHB medium, grown overnight in an Incubator and a incubating shaker at 37oc respectively. Housekeeping gene were identified through online bioinformatics and blasted against known sequences. A set of 6 primers, comprising 2 outer primers (F3 and B3), 2 inner primers (FIP and BIP), and 2 loop primers (FLP and BLP), were designed. Microbial DNA extraction was done followed by PCR amplification as a classical identification using LAMP outer primers 9(F3 and B3). LAMP amplicons were detected by real time turbidimetry (LA-500) at 64°C for 40 minutes as well as under UV light with 1.0 μl of 1/10-diluted original SYBR Green I.

Results: LAMP validation against traditional PCR shows a high limit of detection at 10-6ng/µl compared to 10-5ng/µl for PCR. The findings are consistent with outcomes for real time turbidimetric outcomes. Real time LAMP turbidimetric results was cross validated by direct observation through SYBR fluorescence under UV light for positive P. aeruginosa detection through positive amplification.

Conclusion: Thus far, Loop mediated isothermal amplification show significantly high limit of detection comparable to standard PCR, its use in field based diagnosis offers an opportunity for a cheap, reliable and faster method to determine disease trends and therapy approaches. This method can be applied in primary care to enhance accuracy in diagnosis and thereby prompt initiation of mitigation treatment regimens.

Open Access Original Research Article

A Comprehensive Study of Hexanal Formulation on Extending Shelf Life of Custard Apple (Annona squamosa L.)

Babita Jangir, Shalini Pilania, Vinod Saharan, S. S. Lakhawat, Damyanti Prajapat, Mahendra Meena, Pinki Yadav, Jitendra Kumar Tak

Current Journal of Applied Science and Technology, Page 30-37
DOI: 10.9734/cjast/2021/v40i2631523

Shelf life of custard apple is limited due to climacteric nature. In the present study, two application methods along with different concentration of hexanal formulations were used, first hexanal vapour at different concentrations (0.005, 0.01, 0.02, 0.05% v/v) and second hexanal dip (0.5, 1, 1.5, 2% v/v) along with control were evaluated on custard apple dipping fruits for 2 min and 4 min and store at ambient temperature (27±2ºC). Hexanal filter paper dip in 0.05% concentration effectively maintained the firmness, total sugar, reducing sugar, preserved L*, a*, b* value, extend shelf life and maximum BC ratio during storage from day 1 to days 14 as compare to control. Therefore, we concluded that hexanl vapour(0.05%) was effective to use post harvest technology for custard apple.