Open Access Short Research Article

Correlation and Multiple Regression Studies of Yield and Yield Contributing Characters in Cauliflower (Brassica oleracea var. Botrytis L.)

K. P. Singh, B. Patel, Rakesh Kumar, R. K. Roy, S. K. Singh

Current Journal of Applied Science and Technology, Page 1-5
DOI: 10.9734/cjast/2019/v33i330068

The study on Cauliflower cv. ‘Pusa Dipali’ was carried out to find out the correlation and multiple regression coefficients studies of yield and yield contributing characters. Yield was found to be highly and significantly positively correlated with all the ancillary characters viz, curd depth (0.9180), curd diameter (0.9050), weight of curd (0.8990, plant height (0.8898), weight of plant (0.8768) and plant girth (0.6880). The multiple regression coefficients were found to be non significant due to multi collinearly between the characters. The step wise regression analysis showed that curd depth has highest contribution towards field followed by curd weight, curd diameter and plant height while the lowest contribution was due to plant girth and weight of plant.

Open Access Short Research Article

Constraints Perceived in Following Dairy Animal Welfare Practices in Jamtara District of Jharkhand

Bhushan Kumar Singh, J. Oraon, Alok Kumar Pandey, Rajesh Kumar, Nandani Kumari, Kumari Shweta

Current Journal of Applied Science and Technology, Page 1-4
DOI: 10.9734/cjast/2019/v33i330069

The development of welfare assessment schemes involves a difficult balancing of practical constraints against the need for scientific validity and reliability. There are lots of constraints in following proper welfare practices at farmer’s level. In view of above the present study was conducted on Jamtara district of Jharkhand, it has 6 blocks and to have a complete study of whole district, all the 6 blocks were selected. From the selected blocks, two villages (one Peri urban and one rural village) were selected randomly. Data were collected through structured interview schedule. it was observed trends in constraints were as follow, lack of money was the most significant constraints as it was perceived by 95 per cent of dairy owners, second was lack of information (91.66%), lack of availability of Veterinary Doctors (88.33%), lack of feed (82.50%), lack of Government facility (80.83%), and no financial gain in following animal welfare practices perceived by 74.16% respondents. In conclusion, the major constraints experienced by the dairy farmers to follow dairy animal welfare practices were lack of money, lack of information and lack of availability of veterinary doctor in vicinity.

Open Access Short Research Article

Phenolic Exudation Control and Establishment of In vitro Strawberry (Fragaria × Ananassa) cv. Chandler

Hidayatullah Mir, Ruby Rani, Feza Ahmad, Awadh Kishor Sah, Shashi Prakash, Vikash Kumar

Current Journal of Applied Science and Technology, Page 1-5
DOI: 10.9734/cjast/2019/v33i330071

The rate of strawberry propagation through conventional technique is quite low and it is difficult to maintain planting material during the summer months under Bihar condition. Further, importing mother plants adds to the production cost. In vitro micro propagation has emerged as a potential alternative for supplying planting material for strawberry. Two type of explants viz., runner tip and nodal segment were used for the study. Phenol exudation was the major problem during establishment which caused death of majority explants. In our experiment, almost no phenolic exudation (+) and maximum percent regeneration for runner tip (55.2 ± 0.52%) and nodal segment (58.1 ± 0.54%) was observed when MS medium was supplemented with ascorbic acid 200 mg per liter. Phenolic exudation was recorded highest (++++) under control when no antioxidants were supplemented. Minimum number of days for runner tips (8.4 ± 0.23) and nodal segments (10.3 ± 0.33) taken for shoot proliferation was observed when MS medium was supplemented with activated charcoal 300 mg and 200 mg per liter, respectively. Though all other antioxidants used in our study including citric acid, PVP and activated charcoal significantly reduced oxidative browning, ascorbic acid was found to be most effective antioxidant in controlling lethal browning during in vitro establishment of strawberry. This protocol has a potential for allowing a large scale multiplication of this important crop.

Open Access Short Research Article

Varietal Evaluation of Different Genotypes of Linseed for Yield Performance in Aurangabad District of Bihar

Praveen Kumar, Rajeev Singh, Ravi Ranjan Kumar, Nityanand ., Dinesh Kumar, R. K. Sohane, A. K. Singh, Sangita Mehta

Current Journal of Applied Science and Technology, Page 1-4
DOI: 10.9734/cjast/2019/v33i330074

Linseed is locally known as Tissi, common Flax and Alsi. It belongs to family Linaceae. It is a rabi oil seed crop. It has medicinal and industrial importance. It is rich source of essential fatty acid (alfa-linolenic acid) known as Omega-3 fatty acid, antioxidants and fibre. The present study was carried out in KVK Aurangabad district of Bihar during the year 2017-2018 on the varietal evaluation of different genotypes of linseed for yield performance. The experiment was laid out at the five farmers field. Four linseed varieties (T1-Ruchi, T2-Sabour Tissi, T3-BRLS-102 and T4-Shekhar) were evaluated and screened for their agronomic performance, Net return, B:C ratio and correlation amongst yield and its contributing traits were calculated. Among the four genotypes, the no. of seeds per plant was found to be maximum BRLS-102 (8.35) in followed by Shekhar (8.25) and Sabour Tissi-1 (7.46) which is at par with Ruchi (6.0). The average yield performance of BRLS-102, Sabour Tissi-1, Shekhar and Ruchi was (10.61q/h), (10.08 q/h), (9.58q/h) and (9.10 q/h) respectively. Among the four genotypes of linseed, the net return and B:C ratio has also found to be maximum with BRLS-102 (24,150), (2.32) followed by Sabour Tissi-1 (22,030), (2.20): Shekhar (20,030), (2.09) and which is at par with the genotype Ruchi (18,110)(1.99).

The new genotypes (BRLS-102) & Sabour Tissi-1 possess the potential to become future varieties for cultivation in Aurangabad district of Bihar.

Open Access Short Research Article

Study on Different Methods of Weed Management in Onion (Allium cepa L.)

Sheela Barla, R. R. Upasani

Current Journal of Applied Science and Technology, Page 1-7
DOI: 10.9734/cjast/2019/v33i330079

A weed management study on onion (Allium cepa L.) was conducted at Birsa Agricultural University, Jharkhand, India during winter season of 2016-17 and 2017-18 in a randomized block design comprised of nine weed control treatments consisting plastic mulch, available weeds mulch, straw mulch, cover crops (Fenugreek), oxyfluorfen 0.5 kg/ha pre-emergent (PE), pendimethalin 1.0 kg/ha PE, mechanical weeding by Dutch hoe, hand weeding at 20, 40, 60 days after transplanting (DAT) and weedy check. Hand weeding at 20, 40 and 60 DAT was most effective in reducing weed dry matter however it was similar to plastic mulch at all the growth stages of crop during 2016-17, 2017-18 as well as in pooled except at 60 DAT in 2016-17. Hand weeding reduced weed dry matter to the extent of 75.83 and 84.83 percent during 2016-17, 100 and 89.21 percent during 2017-18, 98.64 and 88.62 percent under pooled data at 30 and 60 DAT respectively and the corresponding reduction in weed dry matter due to application of plastic mulch was 70.73 and 40.32 percent during 2016-17, 100 and 93.64 percent during 2017-18, 98.37 and 86.52 percent under pooled data at 30 and 60 DAT respectively, compared to weedy check. Application of plastic mulch recorded 300 and 38 percent higher onion yield during 2016-17, 1959 and 117 percent during 2017-18, 576 and 70 percent higher under pooled data compared to weedy check and hand weeding respectively. Plastic mulch also recorded maximum net return 364136, 380185 and 372160 /ha during 2016-17, 2017-18 and under pooled data similar to net return with application of oxyfluorfen 0.25 kg/ha PE, pendimethalin 1 kg/ha PE and hand weeding during 2016-17 while under pooled data maximum B:C ratio was with plastic mulch and was similar to oxyfluorfen 0.25 kg/ha PE, pendimethalin 1 kg/ha PE and hand weeding.

Open Access Short Research Article

Study on In vitro Establishment and Callus Induction in Banana cv. Grand Naine

Ravi Kumar, M. Feza Ahmed, H. Mir, Sangita Mehta, R. K. Sohane

Current Journal of Applied Science and Technology, Page 1-5
DOI: 10.9734/cjast/2019/v33i330073

Banana is conventionally vegetatively propagated through suckers, which grow from lateral buds originating from corms and suckers. This process is very slow as the rate of multiplication of suckers through conventional vegetative means has been found to express several negative impacts which include transmission of diseases, low production and poor conservation of original plant genetic material. The rapid proliferation obtained in tissue culture allows nursery men to meet unexpected demand for a particular variety. Sword Suckers of cultivar Grand Naine were used as explants in our study. Contamination is the most severe problem encountered in vitro culture establishment. Mercuric chloride alone and in combination with 70% ethanol at different time duration was used in the study for the establishment of the cultures. The contamination significantly decreased with increase in concentration of sterilants and their time of exposure. The percent of establishment of explants was recorded highest (70.0% ± 2.40) after four weeks of culturing when explants were treated with ethanol (70%) for 30 seconds + HgCl2 (0.1%) for 25 mins.MS media supplemented with 2,4-D 2.0 mg /l+ NAA 0.5 mg/l was found most effective for maximum percentage of callus formation (70.0% ± 1.00). Finally, regeneration of plantlets was achieved on MS medium supplemented with 3.0 mg/l BAP and 1.0 mg/l NAA. Our results described various factors that influence the in vitro establishment and callus formation of banana cv. Grand Naine.

Open Access Short Research Article

In vitro Multiplication and Genetic Fidelity Studies in Cleopatra Mandarin (Citrus reshni Tanaka)

Shashi Prakash, Suneel Sharma, Subhash Kajla, Renu .

Current Journal of Applied Science and Technology, Page 1-6
DOI: 10.9734/cjast/2019/v33i330077

The present investigation was undertaken for in vitro multiplication of Cleopatra mandarin using nodal segment explants. Nodal segments taken from 9 years old plant in the month of March - April were cultured on Murashige and Skoog (1962) medium supplemented with BAP 0.5 mg/l + Kin 0.5 mg/l for establishment. Various concentrations of auxins viz.  IAA and NAA were used singly or in combination with cytokinins (Kinetin and BAP) for shoot multiplication. Maximum number of shootlets per sprouted bud (8.2 ± 0.22) and maximum length of shootlets (3.0 ± 0.12cm) were recorded when auxins were used in combination with cytokinins at a concentration of NAA 0.3 mg/l + BAP 1.0 mg/l. Full strength MS media fortified with 1mg/l IBA was used for rooting of multiplied shoots and was kept in potting media containing sand, soil and vermi compost in 1:1:1 ratio for hardening.In vitro raised plants were examined for genetic stability by using RAPD primers. Out of fifty primers screened, eleven primers produced amplification while thirty-nine primers did not show any amplification. All RAPD profile for in vitro raised plants were monomorphic and similar to their mother plant, which showed that all the plants raised through micropropagation were true to type.

Open Access Minireview Article

Impact of Climate Change in Capsicum Production: A Review

D. Srikanth, G. Kranthi Rekha, A. Praveena Lakshmi, P. Vimatha

Current Journal of Applied Science and Technology, Page 1-5
DOI: 10.9734/cjast/2019/v33i330075

Vegetable crops are sensitive to temperature, most of them require specific temperatures for optimum yield and quality. Climate change will effect capsicum production through increase in pollination failures, floral abortion, reduced fruit size and quality under higher temperature, increased incidence of physiological disorders (sun scald and blossom end rot), increased risk of soil borne diseases (leaf blight and fruit rot). Increasing temperatures will also influence greenhouse crop production, particularly in sub-tropical regions. In temperate areas there will be less effect and sowing time can be adjusted accordingly. Irrigation requirement will be high because of higher evaporative demand. So there is a need to adjust the sowing time according to changing temperatures. Selecting cultivars which are more tolerant are adaptable to changing environmental conditions. So polyhouses are best solution to avoid losses due to unfavourable climatic conditions like high temperature, heavy rains, strong winds and hailstones etc. Integrated pest and disease management (IPDM) will be an important tool to adapt to changing climate. Mulching with different mulching materials will help in reducing the incidence of soil born diseases like fruit rot and leaf blight. Scientists have to breed the cultivars, suitable to grow under changing temperature, resistant to insect, pest and diseases. To cope up with the effects of climate change we have to follow good production technology in capsicum and also to increase the yield and for good quality of the produce.

Open Access Original Research Article

Outdoor Hatchery Larval Biology and Seed Production of Ganga River Prawn Macrobrachium gangeticum (Bate)

Suday Prasad

Current Journal of Applied Science and Technology, Page 1-7
DOI: 10.9734/cjast/2019/v33i330066

Macrobrachium gangeticum (Bate) the third largest freshwater prawn in habitat in the river Ganga and Brahmaputra, which drain into Bay of Bengal. Seed production of this species (M. gangeticum) in India is done in indoor hatcheries. An outdoor hatchery system has been developed by the author at ICAR Research Complex for Eastern Region, Patna, in which M. gangeticum post larvae are produced. Larval rearing trials for M. gangeticum were carried out during the year 2008. The hatchery shades were covered with non transparent polythene sheets at the roof top to avoid direct sunlight. Larvae were reared in brackish water of 8-14 ppt (part per thousand) salinity for growth and development. Ten thousand larvae were stocked in 300 L tank and fed with live nauplii of Artemia salina twice in a day and after that 1 or 2 days, green algae developed in the larval rearing tank due to open sunlight, these algae were found to be ingested by larvae. The larvae fed voraciously and grew faster because of availability of green algae in the larval rearing tanks. The larvae passed through 15 molts, showing the characteristics of 11 distinct larval stages. First occurrence of post larvae (PL) has been recorded within 20 days and trial was conducted on the 35 days. The water quality parameters viz. water temperature, salinity, pH, dissolved oxygen; total hardness and alkalinity were recorded. Production of PL in trials during rearing was 6,480 and 5,870 with 21.6 and 19.56 PL/L respectively. The result of the present trials, the post larval production and shorter larval duration in M. gangeticum indicated the potential of commercial culture and hatchery operation in inland region.

Open Access Original Research Article

Optimization of High Density Planting with Drip Irrigation in Rabi Onion

M. D. Ojha, Vijay Kumar, R. B. Verma, M. Pal, S. R. P. Singh, P. K. Singh

Current Journal of Applied Science and Technology, Page 1-6
DOI: 10.9734/cjast/2019/v33i330067

Irrigation scheduling is necessary for increasing onion production in an Agro-climatic zone III A and B of Bihar, where water is becoming scarce gradually and a limiting factor for onion production. Field experiments were conducted during the Rabi season of 2013-14 and 2014-15 at the experimental farm, Nalanda College of Horticulture, Noorsarai, Nalanda. The objectives were to standardize high density planting with drip irrigation and to study the yield performance and storability on onion in Nalanda region. The treatments consisted of factorial combination of three irrigation intervals (2, 4 and 6 days) and four population densities (2000000, 1333333,  1000000 and 666666 plants/ha) corresponding to 10 x 5, 10 x 7.5, 10 x 10 and 15 x 10 cm respectively. The experiments were laid out in randomized block design replicated three times. Results revealed that the highest marketable yield was significantly favoured by 2 days interval followed by 4 days. However, lower plant spacing 10 x 5 cm recorded higher plant height, minimum maturity days, grass and marketable bulb yield were significantly higher with plant spacing 10 x10 cm followed by 15 x10 cm. The marketable bulb yield was significantly higher with interaction of T7 (10 x10 cm with 2 days irrigation interval) 1000000 plant density and 2 days irrigation interval than other treatment combinations. From the results of the study, it can be concluded that 2 days interval irrigation with a plant density of 1000000 (10x10 cm) plants per hectare can be practised for maximum onion bulb yield.

Open Access Original Research Article

Genetic Diversity Analysis in Grasspea (Lathyrus sativus L.) Using SSR Molecular Markers

Soni Kumari, Jitesh Kumar, Ravi Ranjan Kumar, Anand Kumar, M. S. Nimmy, Tushar Ranjan, Vinod Kumar

Current Journal of Applied Science and Technology, Page 1-6
DOI: 10.9734/cjast/2019/v33i330070

The study of genetic diversity among Lathyrus sativus L. may give fundamental insights into extent of genetic variation and provide options to meet the climate change challenge. 20 SSR loci were employed to assess the genetic diversity of 32 grasspea genotypes. Eleven markers proved to be polymorphic across examined genotypes in aggregation to allow detection of a total of 21 alleles with an average of 1.91 alleles per locus. The polymorphism information content (PIC) calculated as a relative measure of informativeness for each of the SSR markers, ranged from 0.11 to 0.34. The dendrogram from the neighbour-joining UPGMA cluster analysis of the pair-wise simple matching dissimilarity coefficients matrix grouped genotypes into three main clusters. Therefore the characters contributing maximum to genetic diversity may be given importance during grasspea hybridization program and these genotypes may be utilized in further grasspea breeding program aimed to enhance grain yield.

Open Access Original Research Article

Diallel Analysis for Estimation of Combining Ability for Seed Yield and It’s Component Traits in Linseed (Linum usitatissimum L.)

Divya Mahto, Anshuman Vashistha, Suparna Sinha, P. K. Singh, Shailesh Marker

Current Journal of Applied Science and Technology, Page 1-9
DOI: 10.9734/cjast/2019/v33i330072

The present investigation consisted of eight parents of linseed which were crossed as per diallel analysis [1] (Model 1 and Method 2) in Rabi 2010-11 to generate 28 crosses (excluding reciprocals). These 28 crosses were evaluated along with eight parents and three checks viz: T-397, Neelum and Allahabad Local in RBD having three replications during 2011-12 at the Field Experimentation Centre of Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad. The data were recorded on ten characters to study the combining ability. The significant mean sum of squares for all the ten characters indicated the presence of a substantial amount of variability. Per se performance for seed yield and its components depicted that cross M-42 (169) × POLF-19 (1765) was found to be best. Analysis of variance for combining ability showed significant differences for all the characters. The parents FRW-6 (973) and POLF-19 (1765) were good general combiner for seedyield and most of the traits. Highest positive SCA effect for seed yield per plant was depicted by the cross PbD2-42 (2789) × GS-129 (1018).

Open Access Original Research Article

Vegetable Grafting: A Multiple Crop Improvement Methodology

T. Lakshmi Tirupathamma, C. Venkata Ramana, L. Naram Naidu, K. Sasikala

Current Journal of Applied Science and Technology, Page 1-10
DOI: 10.9734/cjast/2019/v33i330076

Grafting is a simple method of propagation in which desired rootstocks are obtained to induce vigor, precocity, enhanced yield and quality, better survival under biotic and abiotic stress conditions. Grafting reduced the dependency upon chemicals required to treat the soil borne diseases and has opened new vista in organic farming of vegetables. Grafting is the popular technology among vegetable growers and researchers to develop resistance in the crops or improve tolerance to biotic and environmental stresses in the various crops mentioning solanaceous and cucurbitaceous crops. The technology of grafting is potential in promoting the cultivation of vegetables in customized and fragile agricultural-ecosystem. It is a alternative tool which is rapid in case of relatively slow breeding methodology and useful in sustainable horticulture which takes low input for future agriculture system. Further, inventions in mechanized and robotic grafting have given a fillip to this novel eco-friendly approach. Mechanization will considerably reduce the cost of grafted seedling production in the future.

Open Access Original Research Article

Effect of Date of Planting and Plant Geometry on Growth and Yield Characters of Kalmegh cv. CIM Megha

Mahender Pal, Vijay Kumar, A. P. Singh, R. S. Singh, M. D. Ojha, S. R. P. Singh, P. K. Singh

Current Journal of Applied Science and Technology, Page 1-5
DOI: 10.9734/cjast/2019/v33i330078

The experiment was laid out in Factorial Randomized Block design assigning time of planting (Factor A), planting geometry (Factor B) with four replications to assess the effect of date of planting and planting geometry on growth and yield of Kalmegh cv. CIM Megha. The experiment was conducted at Experimental farm, Nalanda College of Horticulture, Noorsarai, Nalanda (Bihar Agricultural University, Sabour) in the year 2013-14 and 2014-15. Factor A consisted of three time of planting viz., 15th May, 01st June and 15th June and factor B consisted of two levels of spacing S1 (30 x15 cm) and S2 (30 x20 cm). Pooled data on different growth and yield characters revealed 15th June as  best time of planting of Kalmegh and proclaimed highest values for plant height (75.46 cm), Plant girth (7.69 mm), no. of secondary branches, no. of leaves per plant and total dry matter yield (31.75 q/ha) . on the other hand spacing level of  S2 (30x20 cm) also recorded highest values for plant height (68.75 cm), plant girth (7.75 mm), no. of secondary branched/plant, no. of leaves/plant and total dry matter yield (27.38 q/ha). The treatment combination of 15th June as time of planting and plant spacing of 30 x20 cm found best for planting of Kalmegh in the growing conditions of the Nalanda. It was followed by planting time 15th June and plant spacing of 30x 15 cm for higher growth and yield of Kalmegh.

Open Access Review Article

Phenomics: Approaches and Application in Crop Improvement

P. Roshni, K. A. Prajwala

Current Journal of Applied Science and Technology, Page 1-10
DOI: 10.9734/cjast/2019/v33i330080

Phenotype is the combination of genotype and environment where the plant grows. Phenomics is a way of speeding up phenotyping with the help of high-tech imaging systems and computing power. It has been a practice in plant breeding for selecting the best genotype after studying phenotypic expression in different environmental conditions and also using them in hybridization programs, to develop new improved genotypes. Phenomics share the advantages of faster evaluation, facilitating a more dynamic whole-of-lifecycle measurement with improved precision, being less dependent on periodic destructive assays and with reduced need for replication in the field. Phenomics aids to obtain high-dimensional phenotypic data on an organism at large scale with the various tools involved. Phenomics, however is more than just data collection paired with data mining. It is a comprehensive approach that combines systems biology and statistical correlation. Data mining techniques and big data approaches have the power to create knowledge that cannot be created otherwise with accurate precision manually.