Distribution of Two Species of Begomoviruses Infecting Blackgram in Andhra Pradesh

Main Article Content

B. H. Chaithanya
B. V. Bhaskara Reddy
L. Prasanthi
R. Sarada Jayalakshmi Devi


Background: Yellow mosaic diseases of blackgram is caused by two species of begomoviruses (MYMV, MYMIV). It is known fact that certain varities which are resistant to one species are susceptible to another species when screened by agroinoculations. Hence data on distribution of DNA-A &B components of MYMV / MYMIV under field conditions were not available in Andhra Pradesh.

Methods: In the present study total eighty yellow mosaic infected blackgram samples were colleted from seven districts of Andhra Pradesh during rabi and kharif   2016-17. The total DNA was isolated and detected for presence DNA-A and B components of   MYMV/ MYMIV by PCR with specific primers. Samples which showed negative in normal PCR are further detected by RCA-PCR.

Results: PCR results showed that out of 80 YMD infected blackgram samples tested from seven districts, positve results were otained in 50% samples for MYMV-A, 98.75% for MYMIV-A,and  90% for MYMV-B   and none for MYMIV-B. However RCA-PCR detected MYMIV-B presence in all 7 samples (8.25%) collected from Guntur district, but none from other six districts. These results clearly shows that both species of begoviruses are present in Andhra Pradesh and MYMIV-A is predominant species   in Andhra Pradesh as compared to MYMV-A. Hence germplasm and breedig material should be screened for both the species of begomovirses either under field or through agroinoculations to develop YMV resistant blackgram. 

Blackgram, mungbean yellow mosaic virus, mungbean yellow mosaic India virus, rolling circle amplification, begomoviruses.

Article Details

How to Cite
Chaithanya, B. H., Reddy, B. V. B., Prasanthi, L., & Devi, R. S. J. (2020). Distribution of Two Species of Begomoviruses Infecting Blackgram in Andhra Pradesh. Current Journal of Applied Science and Technology, 39(36), 12-17. https://doi.org/10.9734/cjast/2020/v39i3631068
Original Research Article


Nariani TK. Yellow mosaic of mung (Phaseolus aureus L.). Indian Phytopathology. 1960;13:24‐29.

Qazi J, Ilyas M, Mansoor S, Briddon R. Legume yellow mosaic viruses: Genetically isolated begomoviruses. Molecular Plant Pathology. 2007;8:343–348.

Mishra GP, Dikshit HK, Ramesh SV, Tripathi K, Kumar RR, Aski M, Singh A, Roy A, Priti Kumari N, Dasgupta U, Kumar A, Praveen S, Nair RM. Yellow mosaic disease (YMD) of mungbean (Vigna radiata (L.) Wilczek): Current status and management opportunities. Frontiers in Plant Science. 2020;11:1-24.

Usharani KS, Surendranath B, Haq QMR, Malathi VG. Infectivity analysis of a soybean isolate of Mungbean yellow mosaic India virus by agroinoculation. Journal of General Plant Pathology. 2005; 71:230-237.

Karthikeyan AS, Vanitharani R, Balaji V, Anuradha S, Thillaichidambaram P, Shivaprasad PV, Parameswari C, Balamani V, Saminathan M, Veluthambi K. Analysis of an isolate of mungbean yellow mosaic virus (MYMV) with a highly variable DNA-B component. Archives of Virology. 2004;149:1643-1652.

Haq QMI, Rouhibakhsh A, Ali A, Malathi VG. Infectivity analysis of a blackgram isolate of Mungbean yellow mosaic virus and genetic assortment with MYMIV in selective hosts. Virus Genes. 2011;42: 429-439.

Reddy BVB, Prasanthi L, Shareef SM, Jayalakshmi Devi RS. Duplex PCR for detection of two species of begomoviruses associated with yellow mosaic diseases (YMD) of blackgram in Andhra Pradesh. XXIII National Conference-VIROCON. 2014;30:97.

Reddy BVB, Obaiah S, Prasanthi L, Sivaprasad Y, Sujitha A, Krishna TG. Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India. Archives of Phytopathology and Plant Protection. 2015;48(4):345-353.

Satya VK, Alice D, Malathi VG, Velazhahan R. Molecular identification and genetic diversity of yellow mosaic virus in blackgram. Biochemical and Cellular Archives. 2015;15(2):475-479.

Murray MG, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. 1980;8:4321-4326.

Packialakshmi RM, Srivastava N, Girish KR, Usha R. Molecular characterization of a distinct begomovirus species from Vernonia cinerea and its associated DNA-beta using the bacteriophage Phi 29 DNA polymerase. Virus Genes. 2010; 41:135–143.

Karthikeyan A, Sudha M, Pandiyan M, Senthil N, Shobana VG, Nagarajan P. Screening of MYMV resistant mungbean (Vigna radiate L. Wilczek) progenies through agroinoculation. International Journal of Plant Pathology. 2011;2(3):115-125.

Rocha KCG, Marubayashi JM, Navas-Castillo J, Pavan MA, Krause-Sakate R. Occurrence and genetic variability of Tomato severe rugose virus in pepper and tomato plants in São Paulo State. Summa Phytopathol. 2010;36:222-27.

Jeevalatha A, Singh BP, Priyanka Kaundal, Ravinder Kumar, Baswaraj Raigond. RCA-PCR: A Robust technique for the detection of tomato leaf curl new delhi virus-Potato at ultra low virus titre. Journal of Potato. 2014;41(1):76-80.

Chaithanya BH, Reddy BVB, Prasanthi L. RCA-PCR assay is more effective than traditional PCR assay for detecting begomovirus in greengram. Environment and Ecology. 2017;35(3):1932-1935.

Kumar S, Bhaben T, Sunil KM, Lingaraj S. Molecular characterization and infectivity of Mungbean yellow mosaic India virus associated with yellow mosaic disease of cowpea and mungbean. Biocatalysis and Agricultural Biotechnology. 2017;11:183-191.

Archana S, Venkatesh, Padmaja AS, Manjunatha N, Nagaraju N. Molecular detection and characterization of yellow mosaic virus (YMV) infecting blackgram (Vigna mungo L.) in Karnataka with the aid of coat protein (cp) gene. Journal of Food Legumes. 2017;30(4):277-280.

Nair RM, Gotz M, Winter S, Giri RR, Boddepalli VN, Sirari A, Bains TS, Taggar GK, Dikshit HK, Aski M, Boopathi M, Swain D, Rathore A, Anil Kumar V, Lii EC, Kenyon L. Identification of mungbean lines with tolerance or resistance to yellow mosaic in fields in India where different begomovirus species and different Bemisia tabaci cryptic species predominate. European Journal of Plant Pathology. 2017;149(2): 349-365.