Main Article Content
Aims: The aim of the present work was to generate complete panicle emergence mutants of Samba Mahsuri (BPT-5204), using the chemical mutagen, ethyl methane sulphonate (EMS)
Place and Duration of Study: The field evaluation was carried out repeatedly in two locations at Indian Institute of Rice Research, Rajendranagar and at ICRISAT Patancheru Hyderabad. The duration of the study was three successive seasons in triplicates namely, june-2014, january-2015 and june-2015.
Methodology: Seedlings of 28-30 days old were transplanted in 5 lines (each line containing 20 plants) into the field. The plant spacing was 20 cm by 15 cm with density of one hill. The field was irrigated throughout the experiment with average of 10 cm water above the soil level. Regular hand weeding was embarked upon to free the plant of inter specific competition. The phenotypic data was recorded for CPE mutants through visual assessment. The characteristics that required measurements were done according to the usual procedure. The Distinctness, Uniformity and stability of the CPE mutant lines have been recorded following (DUS) test guidelines by UPOV guidelines for rice (38) & Yoshida S (44).
Results: The identified stabilized thirteen complete panicle emergence (CPE) mutants showed superior agro morphological characters, compared with wild type (BPT-5204). The mutants CPE-2 and CPE-3 took 100 days to flowering and CPE-4 and CPE-7 mutants showed superiority for panicle length, and CPE-5 exhibited good performance for grain yield. In correlation analysis CPE trait had a significantly positive correlation with PH and TGW and negative correlation with 50%DFF. The CPE mutants used in the present study exhibited variability for most of the studied traits. Significant variation was detected in the identified stabilized CPE mutants for plant height, panicle length, flag leaf length and width, 50% flowering, different grain types and 1000 grain weight.
Conclusion: The present study discovered adequate genetic divergence in the thirteen stabilized CPE mutants for various qualitative and quantitative traits. The promising mutants identified during the current study have the potential to be used in future breeding programs for getting productive and quality results.
Rana, Md Masud, et al. Genetic diversity analysis of NERICA lines and parents using SSR markers. Int. J. Plant Soil Sci. 2018;23(6):1-10.
Sasaki A, et al. A mutant gibberellin-synthesis gene in rice. Nature. 2002; 416(6882):701-702.
Suman K et al. Variation of grain quality characters and marker-trait association in rice (Oryza sativa L.); 2019.
Yuan LP, Virmani SS. Status of hybrid rice research and development. Hybrid rice. 1988;7-24.
Mohapatra T, et al. EMS induced mutants of upland rice variety Nagina22: generation and characterization. Proceedings of the Indian National Science Academy. 2004; 80(1):163-172.
Feng et al. 2002; Goff et al., 2002; Sasaki et al., 2002; Yu et al.; 2002.
DES, DACFW; 2016.
FAO (Food and Agriculture Organization) Rome; 2017.
Mohanty, Samarendu, et al. Rice and climate change: Significance for food security and vulnerability. International Rice Research Institute. 2013;14.
Fu, Hao-Wei, You-Fa Li, Qing-Yao Shu. "A revisit of mutation induction by gamma rays in rice (Oryza sativa L.): implications of microsatellite markers for quality control. Molecular Breeding. 2008;22(2):281-288.
Yoshida, Shouichi. Fundamentals of rice crop science. Int. Rice Res. Inst; 1981.
Ashraf M. Inducing drought tolerance in plants: Recent advances. Biotechnology Advances. 2010;28(1):169-183.
Alcantara TP, Bosland PW, Smith DW. Ethyl methanesulfonate-induced seed mutagenesis of Capsicum annuum. Journal of Heredity. 1996;87(3):239-241.
Bhat TARIQ A, Khan AH, Parveen SAHBA. Comparative analysis of meiotic abnormalities induced by gamma rays, EMS and MMS in Vicia faba L. J Indian Bot Soc. 2005;84:45-48.
Botticella E, Sestili F, Hernandez-Lopez A, Phillips A, Lafiandra D. High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes. BMC Plant Biol. 2011;11:15610. 1186/1471-2229-11-156.6.
VRK Reddy. Cytogenetic effects of gamma rays and ethyl methane sulphonate in chilli pepper (Capsicum annuum L.). Cytologia. 2000;65(2):129-133.
Koornneeff M, Dellaert LWM, Van der Veen JH. EMS-and relation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 1982;93(1): 109-123.
GM Rao. Efficiency and effectiveness of gamma rays and EMS in rice. Cytologia. 1977;42(3-4):443-450.
Potupureddi, Gopi, et al. Identification of agro-morphological characters in sheath blight tolerant lines of Samba mahsuri (BPT-5204) rice variety. Bull. Environ. Pharmacol. Life Sci. 2017;6:41-45.
Zhan, Chengfang et al. Genome-wide association analysis of panicle exsertion and uppermost internode in rice (Oryza sativa L.). Rice. 2019;12(1):72.
Zhao CF, et al. Analysis of QTLs for panicle exsertion and its relationship with yield and yield-related traits in rice (Oryza sativa L.). Genet Mol Res. 2016;15(2):gmr-15027423.
UPOV Guidelines for rice (Oryza sativa L.), TG 16/4. 1985;2-18.
Mao Hailiang, et al. Linking differential domain functions of the GS3 protein to natural variation of grain size in rice. Proceedings of the National Academy of Sciences. 2010;107(45):19579-19584.
Slaton N. Rice production handbook. University of Arkansas, United States Department of Agriculture; 2011.
Shivani D, et al. Character Association Analysis for Yield, Yield Attributes in Swarna X Type 3 Ril Population of Rice; 2018.
Sangeetha A, et al. Statistical package for agricultural research (SPAR 2.0). J. Indian Soc. Agric. Stat. 2008;62:65–74.
Aslam Sagar M, Akram M, Ashraf M. Agronomic and quality traits of some elite rice genotypes. Pakistan Journal of Scientific and Industrial Research. 1995; 38:348-350.
Ali SS, et al. Heritability of yield and yield components of rice. Pakistan Journal of Agricultural Research. 2000;16(2);89-91.
Cheema AA, Awan MA, Iqbal J. Improvement of plant height architecture in basmati rice. Pakistan Journal of Agricultural Research (Pakistan); 1987.
Hori, Kiyosumi, et al. Variation in heading date conceals quantitative trait loci for other traits of importance in breeding selection of rice. Breeding Science. 2012; 62(3):223-234.
Madhubabu P, et al. Evaluation of grain yield, quality and nutrients content in four rice (Oryza sativa L.) Genotypes. Current Journal of Applied Science and Technology. 2017;1-12.
Dutta RK, MA Baset Mia, Sakina Khanam. Plant architecture and growth characteristics of fine grain and aromatic rices and their relation with grain yield. International Rice Commission Newsletter. 2002;51:51-55.
Kusutani A., et al. Studies on the varietal differences of harvest index and morphological characteristics of rice. Japanese J. Crop Sci. 2000;69:359-364.
Yaqoob M, Hussain N, Rashid A. Assessment of genetic variability in rice (Oryza sativa L.) genotypes under rainfed conditions. Journal of Agricultural Research. 2012;50:311-319.
Tiwari Dinesh K, et al. Studies on genetic variability for yield components in rice (Oryza sativa L.). Advances in Agriculture & Botanics. 2011;3(1):76-81.
Joshi MA, et al. Varietal characterization of rice (Oryza sativa L.) based on morphological descriptors. Seed Research-New Delhi. 2007;35(2):188.
Yolanda JL, Vijendra Das LD. Correlation and path analysis in rice (Oryza sativa). Madras Agricultural Journal. 1995;82:576-577.
Zahid M, Afzal, et al. Correlation and path analysis studies of yield and economic traits in Basmati rice (Oryza sativa L.). Asian J. Plant Sci. 2006;5(4):643-645.
Samonte PB, SO, Wilson LT, McClung AM. Path analyses of yield and yield‐related traits of fifteen diverse rice genotypes. Crop Science. 1998;38(5): 1130-1136.
Mahto RN, Yadava MS, KS Mohan. Genetic variation, character association and path analysis in rainfed upland rice. Indian Journal of Dryland Agriculture Research and Development. 2003;18(2): 196-198.
Yang YaoLong et al. Genetic analysis of culms traits in rice. Molecular Plant Breeding. 2011;9(2):160-168.
Zafar Nabeela, Summaira Aziz, Masood SHAHID. Phenotypic divergence for agro-morphological traits among landrace genotypes of rice (Oryza sativa L.) from Pakistan. International Journal of Agriculture and Biology. 2004;6(2):335-339.
Shishir Sharma*, Amrit Pokhrel, Anup Dhakal, Ankur Poudel. Agro-morphological characterization of rice (Oryza sativa L.) landraces of Lamjung and Tanahun District, Nepal. Department of Agronomy, Plant Breeding and Agriculture Statistics, Institute of Agriculture and Animal science, Tribhuvan University, Lamjung Campus, Lamjung, Nepal; 2020.
Wattoo Javed Iqbal, et al. Study of correlation among yield related traits and path coefficient analysis in rice (Oryza sativa L.). African Journal of Biotechnology. 2010;9(46):7853-7856.
Rajeswari S, Nadarajan N. Correlation between yield and yield components in rice Coryza sativa L. Agricultural Science Digest. 2004;24(4):280-282.
Sürek halil, Necmi beşer. Correlation and path coefficient analysis for some yield-related traits in rice (Oryza sativa L.) under thrace conditions. Turkish Journal of Agriculture and Forestry. 2003;7(2):77-83.
Matsubara, Kazuki, et al. Ehd3, encoding a plant homeodomain finger‐containing protein, is a critical promoter of rice flowering. The Plant Journal. 2011;66(4): 603-612.
Takahashi, Yasuyuki, et al. Variations in Hd1 proteins, Hd3a promoters, and Ehd1 expression levels contribute to diversity of flowering time in cultivated rice. Proceedings of the National Academy of Sciences. 2009;106(11):4555-4560.