Effect of Salinity on Growth and Some Photosynthetic Pigments of Improved Population in Puccinellia ciliata (Poaceae)

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Ilkay Yavas
Yelda Emek
Aydin Unay


Puccinellia (Puccinellia ciliata Bor.) fairly resistant to salinity and used as forage for livestock in China, Australia, and Turkey. In this study, our objective was to determine the effects of salinity on growth and various photosynthetic pigments of an improved population of Puccinellia via recurrent selection. To accomplish this, effects of salinity on seedlings growth of homogenous Puccinellia was examined, one week after emerging of radicle from seeds. Seeds were germinated on Murashige and Skoog (MS) medium with 6% agar. Seedling growth was studied under different levels of NaCl salinity (0, 10, 20, 30, 40 μS/cm). Salinity applications were carried out for 6 weeks. Cultures were maintained in growth chambers at 24±2ºC and 16/8 light/dark conditions. Germination was scored during 2 weeks after culture initiation. The experiment was performed in a completely randomized design with three replicates. Plant growth parameters such as the number of radicle and tillers, maximum radicle and shoot length, plants fresh and dry weights were investigated. Photosynthetic pigments such as total chlorophyll, chlorophyll a, b, chlorophyll a/b ratio, total carotenoid, β- carotene, lutein and neoxanthin were examined. The maximum values for tiller number per plant, the maximum length of shoot and chlorophyll b were found in the 20 µs/cm, while the maximum length of the radicle was recorded at 10 µs/cm NaCl treatment. It was concluded that low salinity levels (10-20 μS/cm) increased seedling growth, while high salinity levels (30 and 40 μS/cm) inhibited the growth significantly. These results indicate that P. ciliata is a promising salt-tolerant and can be grown productively under low to moderate saline conditions between 10-20 µs/cm.

Germination, growth, halophyte, Puccinellia, salinity tolerance

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How to Cite
Yavas, I., Emek, Y., & Unay, A. (2020). Effect of Salinity on Growth and Some Photosynthetic Pigments of Improved Population in Puccinellia ciliata (Poaceae). Current Journal of Applied Science and Technology, 39(1), 64-70. https://doi.org/10.9734/cjast/2020/v39i130481
Original Research Article


Jenkins S, Barret-Lennard EG, Rengel Z. Impacts of waterlogging and salinity on puccinellia (Puccinellia ciliata) and tall wheatgrass (Thinopyrum ponticum): Zonation on saltland with a shallow water-table, plant growth, Na+ and K+ concentrations in the leaves. Plant Soil. 2010;329:91-104.

Haider MS, Ibrahim M, Athar HR, Sarwar G, Tahir MA. Ability of Puccinellia ciliata to grow in a waterlogged saline environment. Agrochimica. 2013;LVII-N.3:279-288.

Munday C, Bettink K. Future farm industries CRC environmental weed risk assessment protocol; 2012.
(Accessed 16 December 2019)

Reddy MP, Vora AB. Changes in pigment composition, hill reaction activity and saccharides metabolism in Bajra (Pennisetum typhoides S and H) leaves under NaCl salinity. Photosynthetica. 1986;20:50-55.

Zhang Q, Rue K, Wang S. Salinity effect on seed germination and growth of two warm-season native grass species. HortScience. 2012;47:527–530.

Soleimannejad Z, Abdolzadeh A, Sadeghipour HR. Beneficial effects of silicon application in alleviating salinity stress in halophytic Puccinellia distans plants. Silicon. 2019;11:1001-1010.

Daba AW, Qureshi AS, Nisaren BN. Evaluation of some Rhodes grass (Chloris gayana) genotypes for their salt tolerance, biomass yield and nutrient composition. Appl. Sci. 2019;9:1-12.

Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plantarum. 1962;15:473-497.

Wellburn AR. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol. 1994;144:307-313.

SPSS for Windows: Base 10.0 Applications Guide. Chicago, Illinois.

Alshammary SF, Qian YL, Wallner SJ. Growth response of four turfgrass species to salinity. Agric. Water Management. 2004;66:97-111.

Sepehry A, Akhzari D, Pessarakli M, Barani H. Studying the effects of salinity stress on the growth of various halophytic plant species (Agropyron elongatum, Kochia prostrata and Puccinellia distans). World Applied Sciences Journal. 2012;16: 998-1003.

Akhzari D, Sepehry A, Pessarakli M, Barani H. Studying the effects of salinity stress on the growth of various halophytic plant species (Agropyron elongatum, Kochia prostrata and Puccinellia distans). World Appl Sci J. 2012;16:998-1003.

Jenkins S. Ecophysiological principles governing the zonation of puccinellia (Puccinellia ciliata) and tall wheatgrass (Thinopyrum ponticum) on saline waterlogged land in South-Western Australia. PhD Thesis. School of Earth and Geographical Sciences. Faculty of Natural and Agricultural Sciences. The University of Western Australia. 2007;162.

Kiani-Pouya A, Rasouli F. The potential of leaf chlorophyll content to screen bread-wheat genotypes in saline condition. Photosynthetica. 2014;52:288–300.

Mala K, Khan TI. Effect of different levels of salinity on growth and photosynthetic pigments of wheat plants (Triticum aestivum L. var. Raj- 3765). Journal of Environmental Science, Toxicology and Food Technology. 2017;11(5):17-20.

Terletskaya N, Zobova N, Stupko V, Shuyskaya E. Growth and photosynthetic reactions of different species of wheat seedlings under drought and salt stress. Period. Biol. 2017;119(1):37-45.
DOI: 10.18054/pb.v119i1.4408

Salama S, Trivedi S, Busheva M, Arafat AA, Garab G, Erdei L. Effects of NaCl salinity on growth, cation accumulation, chloroplast structure and function in wheat cultivars differing in salt tolerance. J. Plant Physiol. 1994;144:241-247.

Hussain T, Iqbal A, Amir I, Swati ZA. Chlorophyll-based secreening for salinity tolerance in wheat genotypes. Journal of Agricultural and Biological Science. 2013;8(8):596-598.

Ashraf MY, Azmi AR, Khan AH, Ala SA. Effect of water stress on total phenol, peroxidase activity and chlorophyll contents in wheat (Triticum aestivum L.). Acta Physiologeae Plantarum. 1994;16: 185-191.

Naqvi SSM, Mumtaz S, Ali SA, Shereen A, Khan AH, Ashraf MY, Khan MA. Proline accumulation under salinity stress. Is abscisic acid involved? Acta Physiol. Plant. 1994;16(2):117-122.

Khan N, Nazar R, Anjum N. Growth, photosynthesis and antioxidant metabolism in mustard (Brassica juncea L.) cultivars differing in ATP-sulfurylase activity under salinity stress. Sci Hortic. 2009;122:455-460.

Shah SH, Houborg R, McCabe MF. Response of chlorophyll, carotenoid and SPAD-502 measurement to salinity and nutrient stress in wheat (Triticum aestivum L.). Agronomy. 2017;7(3):61.

Oludare Agbolade J, David O, Ajiboye A, Kioko J, Jolayemi O, Olawuni I, Ojo M, Akomolafe G, Adekoya M, Komolafe R. Morpho-physiological effect of selenium on salinity-stressed wheat (Triticum aestivum L.). Journal of Biological Research. 2019;92:24-29.

Ben-Abdallah S, Aung B, Amyot L, Lalin I, Lachaal M, Karray-Bouraoui N, Hannoufa A. Salt stress (NaCl) affects plant growth and branch pathways of carotenoid and flavonoid biosyntheses in Solanum nigrum. Acta Physiol. Plant. 2016;38: 72.
DOI: 10.1007/s11738-016-2096-8

Kahrizi S, Sedghi M. Effect of salt stress on grain reserve composition in ten durum wheat cultivars. J Stress Physiol Biochem. 2013;9:113-121.

Norshazila S, Othman R, Jaswir I, Yumi Zuhanis HH. Effect of abiotic stress on carotenoids accumulation in pumpkin plants under light and dark conditions. Int. Food Res. J. 2017;24(Suppl):S387-S394.

Abadia A, Belkhodja R, Morales F, Abadia J. Effects of salinity on the photosynthetic pigment composition of barley (Hordeum vulgare L.) grown under a triple-line-source sprinkler system in the field. J. Plant Physiol. 1999;154:392-400.