Main Article Content
The present study aimed in exploring the performance of primed seeds in enhancing the quality of proso millet. The primed seeds along with nonprimed seeds were evaluated for emergence, cell proliferation in radicle cells using scanning electron microscope and biochemical parameters. Seeds primed with Pseudomonas fluorescens 20% possessing higher germination and anatomical changes observed through scanning electron microscope revealed more cell proliferation which was found to show rapid cell elongation and cell division of the radicle when compared to nonprimed seeds. The biochemical causes responsible for higher invigorative effect were identified as enhanced enzyme activity recorded through α-amylase content, dehydrogenase activity, protein content, lipase activity, antioxidative enzymes like catalase activity, peroxidase activity and superoxide dismutase with lower electrical conductivity, free amino acid and free sugars of the seed leachate. It is concluded in this study that primed seeds of Pseudomonas fluorescens 20% performed better than other treatments through their exhibition of higher emergence, more cell proliferation and enhanced biochemical parameters.
Khalil SK, Mexal JG, Rehman A, Khan AZ, Wahab S, Zubair M, Khalil IH, Mohammad F. Soybean mother plant exposure to temperature stress and its effect on germination under osmotic stress. Pak J Bot. 2010;42(1):213-225.
Rowse HR. Drum priming - A non-osmotic method of priming seeds. Seed Sci & Technol. 1995;24:281-294.
Bennett MAA. The use of biological methods to enhance vegetable seed quality. Seed Technol. 1998;20:198-208.
Iswariya S, Sujatha K, Subhashini R. Enhancement of Seedling Vigour through Bio-priming for Barnyard Millet Var. MDU 1. Int J Curr Microbiol App Sci. 2019; 8(4):2254-2259.
ISTA. International rules for seed testing. International Seed Testing Association, Bassersdorf, Switzerland; 2011.
Paul AK, Erji SM, Sircar SM. Metabolic changes in rice seeds during storage. Ind J Agric Sci. 1970;40(12):1031-1036.
Kittock PA, Law AG. Relationship of seedling vigour to respiration and tetrazolium chloride reduction of germinating wheat seeds. Agron J. 1968;60:286-288.
Ali-khan ST, Youngs CG. Variation in protein content of field peas. Can J Plant Sci. 1973;53:37-41.
Sadasivam S, Manickam A. Estimation of lipase content. In: Biochemical methods. New age international Ltd., New Delhi; 2008.
Aebi M. Catalase in vitro. In: Methods in Enzymology; 1984.
Castillo FI, Penel I, Greppin H. Peroxidase release induced by ozone in Sedum album leaves. Plant Physiology. 1984;74:846-851.
Dhindsa RH, Dhindsa RP. Thorpe: Leaf senescence correlated with increased level of membrane permeability, lipid peroxidation and decreased level of SOD and CAT. J Exp Bot. 1981;32:93-101.
Presley JT. Relationship of protoplast permeability of cotton seed viability and predisposition of seedling disease. Pl Dis Reptr. 1958;42:582.
Misra PS, Mertz ET, Glover DV. Biochemical analysis of total free amino acids. Cereal Chem. 1975;52:844.
Somogyi, M. Note on sugar determination. J Biol Chem. 1952;195:19-23.
McDonald MB. Seed deterioration: Physiology, repair and assessment. Seed Sci & Technol. 1999;27:177-237.
Bharathi R, Vivekananthan R, Harish S, Ramanathan A, Samiyappan R. Rhizobacteria based bioformulations for the management of fruit rot infection in chillies. Crop Protection. 2004;23(6):835-843.
Job D, Capron I, Job C, Corbineau F, Come D. Identification of germination-specific protein markers and their use in seed priming technology. In: Seed biology: Advances and applications. CAB International, Wallingford, UK. 2000;449-459.
Saranya N, Renugadevi J, Raja K, Rajashree V, Hemalatha G. Seed priming studies for vigour enhancement in onion CO onion (5). Journal of Pharmacognosy and Phytochemistry. 2017;6(3):77-82.
Afzal I, Basra SMA, Shahid M, Farooq M, Saleem M. Priming enhances germination of spring maize (Zea mays L.) under cool conditions. Seed Sci & Technol. 2008; 36:97-503.
Patil SK, Janpure SV, Shelar VR, Dumbre AD. Efficacy of insecticides and fungicides on seed germination, insect infestation and seed mycoflora on pearl millet during storage. Seed Res. 2004;32(2):189-192.
Umair A, Ali S, Muhammad MJ, Ali I, Tareen MN. Effect of seed priming on the antioxidant enzymes activity of mungbean (Vigna radiata) seedlings. Pakistan Journal of nutrition. 2012;11(2): 140-144.
Singh DP, Maurya S, Prakash OM, Singh UP. Phenolic composition and antifungal activity of culture filtrate of Leptoxyphium axillatum. Ind Phytopath. 2005;58:143–148.
Mathivanan S, Chidambaram ALA, Sundaramoorthy P, Baskaran L, Kalaikandhan R. The effect of plant growth promoting rhizobacteria on groundnut (Arachis hypogaea L.) seed germination and biochemical constituents. Int. J. Curr. Res. Acad. Rev. 2014;2(9):187-194.
Evangelina SE, Dionisio-Sese ML, Ismail AM. Seed pre-treatment in rice reduces damage, enhances carbohydrate mobilization and improves emergence and seedling establishment under flooded conditions. Oxford J. Life Sci; 2011.
Afzal IF, Munir CM, Basra SMA, Hameed A, Nawaz A. Changes in antioxidant enzymes, germination capacity and vigour of tomato seeds in response of priming with polyamines. Seed Sci & Technol. 2009;37:765-770.