Main Article Content
Strain-selection for the biotechnological application is critical in modern environmental bioremediation process design. In this study, twenty-one rhizobacterial isolates were obtained from the rhizosphere soil of Cyperus sp., Cyperus rotundus, Mariscus alternifolius and Maricus ligularis. Samples were treated using Bushnell-Haas media fortified with Bonny light crude oil plus 1% (v/v) rhizosphere soil from pre-impacted locations in Bodo-Ogoni, Gokana LGA, Rivers state. They were screened and four bacterial isolates were selected on the basis of -2,3 catechol dioxygenase activity and their growth dynamics using the growth function model in XLSTAT v 2019.1.3. Vapour-phase transfer and viable plate count techniques were employed in the determination of microbial dynamics. The order for relative enzyme activity and degradation rates followed Pseudomonas fluorescens > Achromobacter agilis > Bacillus thuringiensis > Staphylococcus lentus. The order for growth range were 7.0-10.5 Log10CFU/ml, 6.2-10.3 Log10CFU/ml, 7.1-10.1 Log10CFU/ml and 6.4-10.2 Log10CFU/ml for Achromobacter agilis > Pseudomonas fluorescens > Bacillus thuringiensis > Staphylococcus lentus. The growth pattern of these isolates fitted into the 5th order polynomial function (y= pr1+pr2*X+pr3*X2+pr4*X3+pr5*X4+pr6*X5) with R2-values of 0.999, 0.998, 0.991,0.999 compares to Gompertz and Asymptotic functions that have the least predictability with R2- values of 0.893, 0.599, 0.869, 0.894 and 0.80, 0.545, 0.829, 0.688 for the four isolates respectively. Enzyme activity of the isolates revealed that the isolates were most active on the 6th day of the study and had a lag phase within the first few hours to a day of the study. Statistical analyses revealed a significant difference using two-way ANOVA; p< 0.001 for both enzyme activity and growth rate. The results underscore the benefits and richness of rhizobacterial flora as rich in enzymatic activity for ecosystem-recovery. Overall, the study has shown the great potential and feasibility for deploying robust biotechnology for the monitoring of environmental media involving hydrocarbon pollution in the Niger Delta.
Van der Meer JR, Sentchilo V. Genomic islands and the evolution of catabolic pathways in bacteria. Current Opinion in Biotechnology. 2003;14:248–254,699.
Varjani SJ. Bioresource technology microbial degradation of petroleum hydrocarbons. Bioresource Technology. 2017;223:277–286.
Xia M, Fu D, Chakraborty R, Singh RP. Department of Plant and Microbial Biology, University of California, Berkeley, CA. Bioresource Technology; 2019.
Abbasian F, Lockington R, Mallavarapu M, Naidu R. A comprehensive review of aliphatic hydrocarbon biodegradation by bacteria. Appl. Biochem. Biotechnol. 2015;176(3):670- 699.
Chakraborty R, O'Connor SM, Chan E, Coates JD. Anaerobic degradation of benzene, toluene, ethylbenzene and xylene compounds by Dechloromonas strain RCB. Appl. Environ. Microbiol. 2005;71(12):8649-8655.
Elzobair KA, Stromberger ME, Ippolito JA, Lentz RD. Contrasting effects of biochar versus manure on soil microbial communities and enzyme activities in an Aridisol. Chemosphere. 2016;142:145–152.
Bas HS, Dindar E, SFOT. Biodegradation variations of soil enzyme activities in petroleum-hydrocarbon contaminated soil. International Biodeterioration. 2015;105: 268–275.
Schloter M, Dilly O, Munch JC. Indicators for evaluating soil quality. Agric. Ecosyst. Environ. 2003;98:255e262.
Brohon B, Delolme C, Gourdon R. Complementarity of bioassays and microbial activity measurement for the evaluation of hydrocarbon-contaminated soil quality. Soil Biol. Biochem. 2001;33: 883e891
Eibes G, Cajthaml J, Moreira MT, Feijoo G, Lema JM. Enzymatic degradation of anthracene, dibenzothiophene and pyrene by manganese peroxidase in media containing acetone. Chemosphere. 2006; 64:408e414.
Abu GO. Process and phenomenal microbiology: How microbes were created to create jobs for mankind. An inaugural lecture by professor Gideon Orkwagh Abu; Department of Microbiology, Faculty of Science, University of Port Harcourt; 2017.
Das N, Chandran P. Microbial degradation of petroleum hydrocarbon contaminants: an overview. Biotechnology Research International. 2011;941810:13.
Pi Y, Meng L, Bao M, Sun P, Lu J. Inter-national biodeterioration & biodegradation degradation of crude oil and relationship with bacteria and enzymatic activities in laboratory testing. International Biodeteri-oration & Biodegradation. 2016;106:106–116.
Haritash AK, Kaushik CP. Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): A review. J. Hazard. Mater. 2009;169(1):1e15.
Juhasz AL, Naidu R. Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: A review of the microbial degradation of benzo [a] pyrene. Int. Biodete. Biodegr. 2000;45(1):57e88.
Van Beilen JB, Funhoff EG. Alkane hydroxylases involved in microbial alkane degradation. Appl. Microbiol. Biot. 2007;74(1):13e21.
Wentzel A, Ellingsen TE, Kotlar HK, Zotchev SB, Throne-Holst M. Bacterial metabolism of long-chain n-alkanes. Appl. Microbiol. Biot. 2007;76(6):1209e1221.
Ajayi AO, Abiola A. Microbial diversity of petroleum polluted soil at ayetoro community in ilaje riverine oil producing areas of Ondo State, Nigeria. Progress in Petrochemical Science. 2018;1(5):1–7.
Olukunle OF. Characterization of indigenous microorganisms associated with crude oil-polluted soils and water using traditional techniques. Microbiology Journal. 2013;3:1–11.
Hassan, Ramadan, Sherif El-kadi, Mostafa Sand. Effect of some organic acids on some fungal growth and their effect of some organic acids on some fungal. International Journal of Advances in Biology. 2015;2(1):1–11.
Amanchukwu SC, Obafemi A, Okpokwasili GC. Hydrocarbon degradation and utilization by a palm wine yeast isolates. FEMS Microbiol. Lett. 1989;57:151-154.
Emmanuel O, Enobong E, Gideon A. Laboratory-scale bioremediation of crude oil polluted soil using a consortia of rhizobacteria obtained from plants in Gokana-Ogoni, Rivers State. Journal of Advances in Microbiology. 2018;9(1):1–17.
Olowomofe T, Oluyege J, Sowole D. Isolation, screening and characterization of hydrocarbon-utilizing bacteria isolated from bitumen-contaminated surface water in Agbabu, Ondo State. Journal of Advances in Biology & Biotechnology. 2017;15(2):1–9.
Shekhar SK, Godheja J, Modi DR. Hydrocarbon bioremediation efficiency by five indigenous bacterial strains isolated from contaminated soils. Int. J. Curr. Microbiol. App. Sci. 2015;4(3):892–905.
Dilmi, Fatiha, Abdelwaheb Chibani, Khadidja Senouci Rezkallah. Isolation and molecular identification of hydrocarbon degrading bacteria from oil-contaminated soil. International Journal of Biosciences. 2017;11(4):272–283.
Odokuma LO. The genius in the microbe: an indispensable tool for the management of xenobiotic mediated environmental flux. Inaugural Lecture Series No. 87, University of Port Harcourt, Nigeria; 2012.
Olukunle OF, Babajide O, Boboye B. Effects of temperature and pH on the activities of catechol 2,3-dioxygenase obtained from crude oil contaminated soil in Ilaje, Ondo State, Nigeria. The Open Microbiology Journal. 2015;9(1):84–90.
Stephen E, Emmanuel OE, Okpanachi OS, Emmanuel S, Temola OT. In vitro study of biodegradation of spent lubricating oil by Aspergillusniger. Nature and Science. 2013;11(10):40-44.
Ijah UJ, Abioye OP. Assessment of physicochemical and microbiological properties of soil, 30 months after kerosene spill. Journal of Research Science Management. 2003;1(1):24-30.
Shaopeng Yan, Qiuyu Wang, Lina Qu, Cong Li. Characterization of oil-degrading bacteria from oil-contaminated soil and activity of their enzymes. Biotechnology & Biotechnological Equipment. 2013;27(4):3932-3938.
Okerentugba PO, Ezeronye OU. Petroleum degrading potentials of single and mixed microbial cultures isolated from rivers and refinery effluent in Nigeria. Journal of Biotechnology. 2003;2:288– 292.
Chikere CB, Ughala E. Preliminary screening of hydrocarbon utilizing bacteria habouring plasmids. TWOWS Afr Int J Sci Tech. 2011;2:26-36.