Main Article Content
Several species of endophytic bacteria has been reported from various plants. In the present study, samples of roots, rhizome, pseudostem, petiole and leaves of healthy Banana plant were collected from variety Yangambi km5 (AAA). In total, 38 endophytic bacteria were isolated. Amongst 16 isolates were selected based on compatibility by cross streak method. The 16 strains tested by paper disc method, Lf4, Lf5, Lf10, Pt4 and Ps7 showed inhibitory effects against Pectobaterium carotovorum subsp. carotovorum under in vitro conditions. The Lf4 and Lf5 showed similar results for the biochemical characteristics studied. The isolate Lf10 showed a slight difference with regard to oxidase test, Methyl red, Urease test and H2S production. The isolates Pt4 and Ps7 showed similar result except nitrate reduction, KOH test and Pigment production. Bacillus subtilis (Bs) strain (culture collected from Department of Plant pathology, TNAU) was used as reference culture. The total DNA extracted from selected five isolates was identified by partial sequencing of the 16S rRNA gene and phylogenetic tree was constructed using MEGA 6.0. The results confirmed that isolates Lf4 and Lf5 were Bacillus subtilis, isolate Lf10 was an Ochrobactrum daejeonense, isolate Pt4 was an Achromobacter xylosoxidans and Ps7 was a Pseudomonas aeruginosa. The study revealed that all the five strains have biocontrol potential against soft rot pathogen.
Bulgarelli D, Schlaeppi K, Spaepen S, Themaat EVLV, Lefert PS. Structure and functions of the bacterial microbiota of plants. Annu. Rev. of Plant Biol. 2013;64:807-838.
Ryan RP, Germaine K, Franks A, Ryan DJ, Dowling DN, Bacterial endophytes: Recent developments and applications. FEMS microbiol. Lett. 2008;278(1):1-9.
Compant SR, Birgit S, Angela N, Jerzy C, Ait BE. Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain PsJN. Appl. Environ. Microbiol. 2005;71(4):1685-1693.
Thomas P. A three-step screening procedure for detection of covert and endophytic bacteria in plant tissue cultures. Curr. Sci. 2004;87(1):67-72.
Thomas P, Sekhar AC. Live cell imaging reveals extensive intracellular cytoplasmic colonization of banana by normally non-cultivable endophytic bacteria. AoB Plants. 2014;12:6.
Sekhar AC, Thomas P. Isolation and identification of shoot-tip associated endophytic bacteria from banana cv. Grand Naine and testing for antagonistic activity against Fusarium oxysporum f. sp. cubense. Am. J. Plant Sci. 2015;6(07): 943.
Jasim B, Sreelakshmi S, Mathew J, Radhakrishnan EK. Identification of endophytic Bacillus mojavensis with highly specialized broad spectrum antibacterial activity. 3 Biotech. 2016;6(2):187.
Aliye N, Fininsa C, Hiskias Y. Evaluation of rhizosphere bacterial antagonists for their potential to bioprotect potato (Solanum tuberosum) against bacterial wilt (Ralstonia solanacearum). Biol. Control. 2008;47(3):282-288.
Kavino M, Harish S, Kumar N, Saravanakumar D, Damodaran T, Soorianathasundaram K, Samiyappan R. Rhizosphere and endophytic bacteria for induction of systemic resistance of banana plantlets against bunchy top virus. Soil Biol. Biochem. 2007;39(5):1087-1098.
Patil VR, Potdukhe SR, Guldekar DD, Ghate AM. Morphological and biochemical characterization of Azotobacter chroococcum from soils of different locations of nagpur district. J. Soils and Crops. 2014;24(1):148-153.
Schaad WN, Jones JB, Chun W. Laboratory guide for the identification of plant pathogenic bacteria: American Phytopathological Society (APS Press). Third edition. 2001;44-58.
Chen WP, Kuo TT. A simple and rapid method for the preparation of gram-negative bacterial genomic DNA. Nucleic Acids Res. 1993;21(9):2260.
Thomas P, Swarna GK, Patil P, Rawal RD. Ubiquitous Presence of Normally Non-Cultivable Culturable Endophytic Bacteria in Field Shoot-Tips of Banana and Their Gradual Activation to Quiescent Cultivable Form in Tissue Cultures. Plant Cell Tiss. Org. 2008;93:39-54.
Thomas P, Swarna GK, Roy PK, Patil P. Identification of Cultivable Culturable and Originally Non- Culturable Endophytic Bacteria Isolated from Shoot Tip Cultures of Banana cv. Grand Naine. Plant Cell Tiss. Org. 2008;93:55-63.
Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, Higgins DG, et al. Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Research. 2003;13:3497-3500.
Schulz B, Boyle C. What are endophytes? In Microbial root endophytes. Springer. 2006;9:1-13.
Thomas P, Soly TA. Endophytic bacteria associated with growing shoot tips of banana (Musa sp.) cv. Grand Naine and the affinity of endophytes to the host. Microb. Ecol. 2009;58(4):952-964.
Tashi‐Oshnoe F,Harighi B, Abdollahzadeh J. Isolation and identification of endophytic bacteria with plant growth promoting and biocontrol potential from oak trees. Forest pathol. 2017;47(5): e12360.
Abd-El-Khair H, Karima HEH. Application of some bactericides and bioagents for controlling the soft rot disease in potato. Res. J. Agric. Biol. Sci. 2007;3(5): 463-473.
Rao MS, Kamalnath M, Umamaheswari R, Rajinikanth R, Prabu P, Priti K, Gopalakrishnan C. Bacillus subtilis IIHR BS-2 enriched vermicompost controls root knot nematode and soft rot disease complex in carrot. Sci. Hortic. 2017;218: 56-62.
Cui W, He P, Munir S, He P, He Y, Li X, He P. Biocontrol of soft rot of Chinese cabbage using an endophytic bacterial strain. Front. Microbial. 2019;10:1471
Hahm MS, Sumayo M, Hwang YJ, Jeon SA, Park SJ, Lee JY, Ahn JH, et al. Biological control and plant growth promoting capacity of rhizobacteria on pepper under greenhouse and field conditions. J. Microbiol. 2012;50:380−385.
Kumaresan V, Suryanarayanan T. Occurrence and distribution of endophytic bacteria in a legume community. J. Bacteriol. 2001;105:1388-1391
Son HJ, Park GT, Cha MS, Heo MS. Solubilization of insoluble inorganic phosphates by a novel salt- and pH tolerant Pantoea agglomerans R-42 isolated from soybean rhizosphere. Bioresearch Technol. 2006;97:204-210.
Cappuccino JG, Sherman N. Microbiology a Laboratory Manual. 6th Edn., Pearson Education, New York. 2002;133-198.
Ouma SO, Mugweru JL, Ngamau CN, Matiru VN. Evaluation of nitrogen fixation ability of endophytic bacteria in Kenyan bananas (Musa Spp.) using biochemical and molecular techniques. The International J. Sci. Tech. 2015;13(2): 156-163.
Thomas P, Sekhar AC, Mujawar MM. Nonrecovery of varying proportions of viable bacteria during spread plating governed by the extent of spreader usage and proposal for an alternate spotting‐spreading approach to maximize the CFU. J. appl. Microbiol. 2012;113(2): 339-350.
Shridhar AM. Development of liquid bioinoculant of endophytic nitrogen fixing bacteria from banana musa spp and its evaluation against mycosphaerella musicola. Pune; 2016.