Efficiency of Bio-fungicides (Trichoderma spp and Pseudomonas fluorescens) on Seedling Emergence, Vigour and Health of Infected Chilli Seeds (Capsicum annuum) by Colletotrichum capsici
Current Journal of Applied Science and Technology,
Damping off and fruit rot caused by Colletotrichum capsici are the major constraints in production and marketability of chilli. Systemic fungicides are commonly used to control this disease. However, continuous use of chemical fungicides leads to negative impact on environment, soil and human health. Therefore, present studies (blotter and pot experiment) were conducted to explore the bio-fungicides (as an alternative to chemical fungicide) in comparison with carbendazim using chilli seeds infected with Colletotrichum capsici.
Experiments were conducted at the CCSHAU, Hisar, India during 2016 in completely randomized design with nine treatments replicated three times. Six months old seeds having germination above the Indian Minimum Seed Certification Standard, were infected with Colletotrichum capsici and such infected seeds were treated with Trichoderma asperellum, Trichoderma viridae, Pseudomonas fluorescens individually and their combinations to control the disease incidence. The infected, un-infected and seed treatment with carbendazim served as controls. Results revealed that the seed germination was significantly higher (94.7%) with Trichoderma viride treatment compared to all other treatments including controls in blotter method. However, the seedling emergence in pot culture was significantly superior with Carbendazim treatment, the seed treatment with Pseudomonas fluorescens and Trichoderma viridae was on par to that of Carbendazim treatment. The seedling length was significantly superior with Trichoderma viride compared to the carbendazim and other controls both in blotter and pot culture. The seedling dry weight and seedling vigour were significantly higher with carbendazim as compared to the Trichoderma viride treatment or other treatments in both blotter and pot culture. However, the overall seedling vigour obtained with Trichoderma viride was similar to that of carbendazim treatment. The disease incidence was significantly lower with Pseudomonas fluorescens as compared to the Trichoderma viride and carbendazim in blotter method and; T. viride + P. fluorescens treatment was on par to that of carbendazim treatment in pot culture. Therefore, use of Trichoderma viride and Pseudomonas fluorescens individually or in combination are suggested as an alternative to carbendazim to control the Colletotrichum capsici.
- Colletotrichum capsici
How to Cite
Pakdeevaraporn P, Wasee S, Taylor PWJ, Mongkolporn O. Inheritance of resistance to anthracnose caused by Colletotrichum capsici in Capsicum. Plant Breeding. 2005; 124(2):206-208.
Pandey KK, Pandey PK. Survey and surveillance of vegetable growing areas for prevalence of major diseases. Vegetable Science. 2003;30(2):128-134.
Phansawan B, Prapamontol T, Thavornyutikarn P, Chantara S, Mangklabruks A, Santasup C. A sensitive method for determination of carbendazim residue in vegetable samples using HPLC-UV and its application in health risk assessment. Chiang Mai Journal of Science. 2015;42(3):681-690.
Avinash VS, Hosmani SP. Effect of carbendazim on morphological and biochemical parameters of Sorghum bicolor. Periplex. Indian Journal of Research. 2012; 1(10): 12-14.
Intana W, Suwanno T, Chamswarng C, Chantrapromma K, Ngamriabsakul C. Increased efficacy for controlling anthracnose of chilli using antifungal metabolites from mutant strains of Trichoderma harzianum. Thai Journal of Agricultural Science. 2007;40(1-2):65-72.
Jeyalakshmi C, Durairaj P, Seetharaman K, Sivaprakasam K. Bio-control of fruit rot and die-back of chilli using antagonistic microorganisms. Indian Phytopathology. 1998;51(2):180-183.
Sharma PN, Kaur M, Sharma OP, Sharma P, Pathania A. Morphological, pathological and molecular variability in Colletotrichum capsici, the cause of fruit rot of chillies in the subtropical region of north-western India. Journal of Phytopathology. 2005; 153(4):232–237.
Srinivas C, Niranjana SR, Kumar P, Chandra L, Nayaka S, Shetty MS. Effect of chemicals and biological agents on seed quality of chilli (Capsicum annum L.). Indian Phytopathology. 2005;59:62-67.
Theerthagiri A, Ramanujam B. Exploitation of plant products and bio-agents for eco-friendly management of chilli fruit rot disease. Journal of Plant Protection Science. 2009;49(2):196-197.
Tiwari PK, Kasyap A, Awadhiya GK, Thrimurty VS. Efficacy of bio-agents, neem based plant products and plant extracts Against Colletotrichum capsici. Indian Journal of Plant Protection. 2008;36(1):97-97.
Sheoran HS, Duhan BS, Grewal KS, Sheoran S. Nitrogen transformation as affected by application of nitrogen, vermicompost and herbicide (Clodinafop Propargyl) in sandy soil. The Bioscan. 2016;11(1):485-490.
Anderson JD, Baki AA. Vigour determina-tion in soybean seed by multiple criteria. Crop Science. 1973;13(6):630-633.
Raj ST, Christopher DJ, Rajakumar RS, Usharani S. Effect of organic amendments and Trichoderma viride on growth and root rot incidence of sunflower. Annals of Plant Protection Sciences. 2008;16:242-244.
Yadav VK. Organic package of practices for chilli from Uttaranchal. Organic Farming Newsletter. 2008;4(4):3-8.
Ekefan EJ, Jama A, Gowen SR. Potential of Trichoderma harzianum isolates in for prevalence of major diseases. Vegetable science. 2009;30(2):128-134.
Rehman SU, Lawrence R, Kumar EJ, Badri ZA. Comparative efficacy of Trichoderma viride, T. harzianum and carbendazim against damping-off disease of cauliflower caused by Rhizoctonia solani. Kuehn Journal of Biopesticides. 2012;5(1):23-27.
Rohini HGG, Hariprasad P, Singh SB, Niranjana SR. Biological control of phomopsis leaf blight of brinjal (Solanum melongena L.) with combining phylloplane and rhizosphere colonizing beneficial bacteria. Biological Control. 2016;101: 123-129.
Jayaraj J, Radhakrishnan NV, Velazhahan R. Development of formulations of Trichoderma harzianum strain M1 for control of damping-off of tomato caused by Pythium aphanidermatum. Achieves of Phytopathology and Plant Protection. 2006;39(1):1-8.
Manoranjitham SK, Prakasan V, Rajappan K, Amutha G. Effect of two antagonists on damping off disease of tomato. Indian Phytopathology. 2000;53(4):441-443.
Asaduzzaman M, Alam MJ, Islam MM. Effect of Trichoderma on seed germination and seedling parameters of chilli. Journal of Science Foundation. 2011;8(1-2):141-150.
Islam MS, Rahman MA, Bulbul SH, Alam MF. Effect of Trichoderma on seed germination and seedling parameters in chilli. Journal of Experimental Agriculture International. 2011;2(1):21-26.
Choudhary CS, Jain SC, Kumar R, Choudhary JS. Efficacy of different fungicides, biocides and botanical extract seed treatment for controlling seed borne Colletotrichum sp. in chilli (Capsicum annuum L.). The Bioscan. 2013;8(1):123-126.
Patel MD, Lal AA, Singh PP. Efficacy of certain bio agents and fungicides against root rot of chilli (Capsicum annum L.). The Bioscan. 2014;9(3):1273-1277.
Ekbote S. Effect of Pseudomonas fluorescens on anthracnose of chilli caused by Colletotrichum capsici. Karnataka Journal of Agricultural Sciences. 2005; 18(1):162-165.
Mesta RK, Mohankumar SA, Aspapupre M, Shivaprasad M, Tatagar MH. Management of fruit rot and powdery mildew of chilli through Pseudomonas fluorescence along with fungicides. National workshop on current trends and future prospects in production and export of spice crops with special to chilli. 2009;123.
Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002;10:178-182.
Abstract View: 2066 times
PDF Download: 736 times