Studies on Mutagenic Effectiveness and Efficiency of Gamma Rays and Ethyl Methane Sulphonate in Jasmine

Main Article Content

Sanchita Ghosh
M. Ganga
K. Soorianathasundaram

Abstract

Background/Aim: The present investigation was undertaken to study the mutagenic effectiveness and efficiency in M1V1 generation and to study effect of gamma rays on spectrum of morphological mutation in Jasminum grandiflorum Linn. cv. White Pitchi.

Methods: Terminal cuttings were treated with four doses of gamma rays viz., 10, 15, 20 and 25 Gy and four doses of EMS viz., 25, 30, 35 and 40 mM separately. Both mutagens created a high frequency as well as a wide spectrum of mutation.

Results: Totally five types of chlorophyll mutants viz., xantha, viridis, yellow viridis, variegata and tigrina were observed. The mutagenic effectiveness and efficiency were calculated based on biological damage as well as chlorophyll mutation frequency on M1 plants. The mutagenic treatments were effective in inducing various types of morphological macro mutants, with few of them showing significant changes in plant height, flowering parameters and flower yield. The lower mutagen doses were associated with higher mutagenic effectiveness and efficiency.

Conclusion: The present study indicated that the physical mutagen gamma rays were more effective and efficient in causing mutations as compared to the chemical mutagen EMS.

Keywords:
Jasminum, mutagen, gamma rays, EMS, effectiveness, efficiency.

Article Details

How to Cite
Ghosh, S., Ganga, M., & Soorianathasundaram, K. (2019). Studies on Mutagenic Effectiveness and Efficiency of Gamma Rays and Ethyl Methane Sulphonate in Jasmine. Current Journal of Applied Science and Technology, 38(6), 1-8. https://doi.org/10.9734/cjast/2019/v38i630414
Section
Original Research Article

References

Jawaharlal M, Thamaraiselvi S, and Ganga M. Packaging technology for export of jasmine (Jasminum sambac ait.) flowers. Journal of Horticultural Science. 2012;7 (2):180-189.

Chayanika S. Morphological and DNA marker-based genetic diversity assessment and tagging qtls controlling economic traits in jasmine (Jasminum spp.). University of Agricultural Sciences, Bengaluru; 2012.

Jo Yeong Deuk, Jin-Baek Kim. Frequency and Spectrum of Radiation-Induced Mutations Revealed by Whole-Genome Sequencing Analyses of Plants. Quantum Beam Sci. 2019;3(7):1-13.

Lamprecht H. Über Blattfarben von Phanerogamen. Klassifikation, Terminologie und Gensymbole von chlorophyll und anderen Farbmutanten. Agri. Hort. Gen. 1960;18:135-168.

Kharakwal MC. Induced mutations for improvement of protein in chickpea (Cicer arietinum L.). Indian J. Genet. 1998;58:61-68.

Konzak C. Efficient chemical mutagenesis, in: The use of induced mutations in plant breeding. Paper presented at the Report of the FAO/IAEA technical meeting organized by the food and agriculture organization of the United Nations and the International Atomic Energy Agency in cooperation with the European Association for Research on Plant Breeding, Rome, Italy; 1965.

Hanafy, Rania Samy, Samia Ageeb Akladious. Physiological and molecular studies on the effect of gamma radiation in fenugreek (Trigonella foenum-graecum L.) plants. Journal of Genetic Engineering and Biotechnology. 2018;16:683–692.

Awan, MA, Konzak C, Rutger J, Nilan R. Mutagenic effects of sodium azide in rice. Crop Science. 1980;20(5):663-668.

Gustafson A. The mutation system of the chlorophyll apparatus. Lunda Guv Asskr MF Adv. 1940;2(11):1-40.

Blixt S, Gelin O, Mossberg R, Ahnstrom G, Ehrenberg L, and Lofgren R. Studies of induced mutations in peas. Ix. Induction of leaf spots in peas. Agr. Hort. Genet. 1964; 22.

Benedict C, Ketring D. Nuclear gene affecting greening in virescent peanut leaves. Plant Physiology. 1972;49(6):972-976.

Nybom, N. On the differential action of mutagenic agents. Hereditas. 1956; 42(12):211-217.

Swaminathan M, Chopra V and Bhaskaran S. Chromosome aberrations and the frequency and spectrum of mutations induced by ethylmethane sulphonate in barley and wheat. Indian Journal of Genetics and Plant Breeding. 1962;22(3): 192-207.

Kolar F, Pawar N, and Dixit G. Induced chlorophyll mutations in Delphinium malabaricum (huth) munz. Journal of Applied Horticulture. 2011;13(1):18-24.

Bhattacharya C. Effect of ethyl methane sulphonate on carnation (Dianthus caryophyllus L.). Environment and Ecology. 2003;21(2):301-305.

Roychowdhury R, Tah J. Assessment of chemical mutagenic effects in mutation breeding programme for M1 generation of carnation (Dianthus caryophyllus). Research in Plant Biology. 2011;1(4).

Padmadevi K, Jawaharlal, M. Molecular characterization of chrysanthemum (Dendranthema grandiflora tzvelev) mutants using RAPD analysis. Asian and Australasian Journal of Plant Science and Biotechnology. 2011;5(1):42 46.

Ambavane AR, Sawardekar SV, Sawantdesai SA, Gokhale NB. Studies on mutagenic effectiveness and efficiency of gamma rays and its effect on quantitative traits in finger millet (Eleusine coracana L. Gaertn). Journal of Radiation and Applied Sciences. 2015;8:120-125.

Mangaiyarkarasi R, Girija M, Gnanamurthy S. Int. J. Curr. Microbiol. App. Sci. 2014; 3(5):881-889.