Enzymatic Clarification and Preservation of Aloe vera Juice by Ohmic Heating

Main Article Content

S. Bansode
R. C. Ranveer
A. R. Tapre
P. M. Ganorkar
S. B. Sadale
A. K. Sahoo


Aims: The aim of this study was to optimize clarification process of the Aloe vera juice followed by its preservation by ohmic heating as no systematic study has been conducted on these aspects.

Study Design: The enzymatic clarification method was used for clarification of Aloe vera juice by using the enzyme pectinase. The enzyme concentration, incubation temperature and time were optimized for clarification of juice. The Aloe vera juice was treated at different Time (min) gradients, current, initial temperature and after temperature at particular current gradient and the ohmic heated juice was then stored in sterilized bottles for further analysis.

Place and Duration of Study: Experiments were done in Department of Food Science and Technology, Shivaji University, Kolhapur, M.S. (India) and completed within 12 months.

Methodology: The optimal conditions for the enzymatic treatment of Aloe vera juice were investigated in order to minimize the turbidity of the juice and maximize the TSS of the juice. The clarified Aloe vera juice was then treated with ohmic heating at different time and current combinations and stored for 60 days to study the physic-chemical and microbial parameters of stored juice.

Results: The recommended enzymatic treatment conditions were: enzyme concentration 1% incubation time 6 h and incubation temperature 45ºC and the TSS, acidity and Turbidity under these conditions were 3.5ºBx, 0.30% and 206.66 NTU respectively. During storage, increased in TSS value from 2.1 to 2.6ºBx, acidity from 0.21 to 0.33% were recorded in ohmic treated juice samples. A very high TPC (102x105 CFU/ml) and yeast and mold count (68x105 CFU/ml) was recorded in untreated sample at 30 days of storage whereas the juice samples treated with ohmic heating at different time and current gradients were observed to be within the limit of standard requirement of microbial quality even up to 60 days of storage.

Conclusion: Enzymatic treatments can reduce the turbidity in Aloe vera juice. Ohmic treatment at different time and current gradients can preserve the clarified juice with respect to its microbial quality for more than 60 days. 

Aloe vera juice, enzymatic clarification, pectinase, ohmic heating.

Article Details

How to Cite
Bansode, S., Ranveer, R., Tapre, A., Ganorkar, P., Sadale, S., & Sahoo, A. (2019). Enzymatic Clarification and Preservation of Aloe vera Juice by Ohmic Heating. Current Journal of Applied Science and Technology, 35(6), 1-9. https://doi.org/10.9734/cjast/2019/v35i630204
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Banjare S, Sharma G, Verma, SK. Potato crop and yield response to different levels of nitrogen under Chhattisgarh plains Agro climatic zone. Indian J Sci Technol. 2014; 7(10):1504-1510.

Lawless J, Allen J. Aloe vera: Natural wonder care. 1st Edn. Thorsons, London: Harper Collins, Hammersmith. 2000;5-12.

Bhargawa Shubhra, Kapoor Swati, Ranote Pushpinder Singh, Sharma Savita. Studies on aloe juice supplemented kinnow nectar. Res. J. Agriculture and Forestry Sci. 2014; 2(8):14-20.

MA Yan Yun, Chang Wen-Wei, Yin Yan, Cao Li-Hua, Yue Zong-Wu. Particle simulation research on the detailed process of bubble generation, Chin. Phys. Lett. 2001;18 (12):1628-1631.

Oana K, Oma Y, Suo S, Takahashi MP, Nishino I, Takeda S, et al. Manumycin A corrects aberrant splicing of Clcn1 in myotonic dystrophy type 1 (DM1) mice. Sci Rep. 2013;3:2142.

Ghaderi S, Kashani-Nejad M, Ghanbari V, Ganjeh M. Three- dimensional simulation and modeling of two phases food system. Iranian Journal of Biosystems Engineering. 2014;45(2):161-167.

Darvishi H, Khoshtaghaza MH, Najafi G. Ohmic heating of pomegranate juice: electrical conductivity and pH change. J. Saudi Soc. Agric. Sci. 2013;12(2):101-108.

A.O.A.C. Official methods of analysis. Association of Official Analytical Chemists. 15th Edition. Washington D.C; 1990.

Munoz OM, Leal X, Quitral V, Cardemil L. Extraction, characterization and properties of the gel of Aloe vera (Aloe barbadensis Miller) Cultivated in Chile. Med Aromat Plants. 2015;4:199.

Sharma R, Tandon D, Joshi VK, Attri S. Development and evaluation of different beverages from Aloe vera (L.) Burm. f. for their nutritional, functional and sensory qualities. Indian Journal of Natural Products and Resources. 2015;6(4):278-282.

Darvishi H, Khoshtaghaza MH, Najafi G. Ohmic heating of pomegranate juice: electrical conductivity and pH change. J. Saudi Soc. Agric. Sci. 2013;12(2):101-108.

Reyes JE, Guanoquiza MI, Tabilo-Munizaga G, Vega-Galvez A, Miranda M, Pérez-Won M. Microbiological Stabilization of Aloe vera (Aloe Barbadensis Miller) Gel by High Hydrostatic Pressure Treatment. Int J Food Microbiol. 2012;158(3):218-224.

Parks LM, Rowe TD. A phytochemical study of Aloe vera leaf. J Am Pharm Assoc. 1941;30(10):262-266.

Femenia A, Sanchez ES, Simal S, Rossello C. Compositional features of polysaccharides from Aloe vera (Aloe barbadensis Miller) plant tissues. Carbohydr. Polym. 1999;39(2):109-117.

Kaur Sukhvir, Harjot Pal Kaur, Parveen Gupta. Physico-chemical analysis of Aloe vera fortified probiotic yoghurt. Int. J. Adv. Pharm., Biol. Chem. 2015;4(2):501-508.

Ranveer RC, Patil SN, Sahoo AK. Effect of different parameters on enzyme-assisted extraction of lycopene from tomato processing waste. Food Biprod. Process. 2013;91(4):370-375.

Poll L. The effect of pulp holding time and pectolytic enzyme treatment on acid content in apple juice. Food Chem. 1993; 47(1):73-75.

Kyamuhangire W, Myhre H, Sorensen HT, Pehrson R. Yield, characteristics and composition of banana juice extracted by the enzymatic and mechanical methods. J. Sci. Food Agric. 2002;82(4):478-482.

Pilink W, Voragen AGJ, Devas L. Enzymic liquefaction of fruits and vegetables. Flussigesn Obst. 1975;42(11):448-451.

Mc Lellan MR, Kime RW, Lind LR. Apple juice clarification with the use of honey and pectinase. J. Food Sci.1985;50:206-208

Choudhari SM, Ananthanarayan L. Enzyme aided extraction of lycopene from tomato tissues. Food Chem. 2007;102:77-81.

Karangwa E, Khizar H, Rao L, Nshimiyimana DS, Foh MBK. Li L, et al. Optimization of processing parameters for clarification of blended carrot-orange juice and improvement of its carotene content. Advance J. of Food Sci and Tech. 2010; 2(5):268-278.

Mabesa, BL, De Lange, RAA, Tan, WT. Extraction of banana juice using commercial pectinase preparation. Philipp. J. Crop Sci. 1989;14:41-44.

Fundira M, Blom M, Pretorius IS, Van Rensburg P. Comparison of commercial enzymes for the processing of marula pulp, wine and spirits. J. Food Sci. 2002; 67(6):2346-2351.

Vikram VB, Ramesh MN, Prapulla SG. Thermal degradation kinetics of nutrients in orange juice heated by electromagnetic and conventional methods. J. Food Eng. 2005;69(1):31-40.

Saeed A, Javid A, Bilal J, Farhat, A. Studies on the preparation and storage stability of pomegranate juice based drink. J. Sci. Res. 2013;16(2):191-195.

Leizerson S, Shimoni, E. Effect of ultrahigh-temperature continuous ohmic heating treatment on fresh orange juice. J. Agric. Food Chem. 2005;53:3519–3524.

Sun H, Kawamura S, Himoto J, Itoh K, Wada T, Kimura T. Effects of ohmic heating on microbial counts and denature-tion of proteins in milk. Food Sci. Technol. Res. 2008;14:117–123.

Ryang JH, Kim NH, Lee BS, Kim CT, Lee SH, Hwang IG, et al. Inactivation of Bacillus cereus spores in a Tsuyu sauce using continuous Ohmic heating with five sequential elbow-type electrodes. J. Appl. Microbiol. 2015;120:175–184.

Uemura K, Isobe S. Developing a new apparatus for inactivating Escherichia coli in saline water with high electric field AC. J. Food Eng. 2002;53:203–207.