Biocontrol of Sclerotium rolfsii Using Antagonistic Activities of Pseudomonads

Main Article Content

Sangita Sahni
Bishun Deo Prasad
Tushar Ranjan

Abstract

Thirty well-characterized pseudomonad isolates for plant growth-promoting traits were screened for their antagonistic activities against 20 isolates of Sclerotium rolfsii.

Out of the 30 pseudomonad isolates, PUR46 was found to be best against all 20 isolates of Sclerotium rolfsii, because of its unique ability to suppress the growth of mycelia as well as the sclerotia formation of most of the S. rolfsii isolates in vitro conditions. In our previous study, PUR46 was also found to be positive for growth promoting traits like phosphorus solubilization and ammonification. The results suggested that expression of one or more of the traits like antagonistic activity against S. rolfsii and solubilization of tri-calcium phosphate may help in controlling the pathogen besides enhancement of plant growth. In this study, our investigations clearly indicate that PGPR isolates PUR 46 may be exploited to be used as potential biocontrol agents against S. rolfsii in agriculture system.

Keywords:
Pseudomonad, Sclerotium rolfsii, plant growth-promoting traits, antagonistic activities.

Article Details

How to Cite
Sahni, S., Prasad, B., & Ranjan, T. (2019). Biocontrol of Sclerotium rolfsii Using Antagonistic Activities of Pseudomonads. Current Journal of Applied Science and Technology, 35(5), 1-9. https://doi.org/10.9734/cjast/2019/v35i530202
Section
Original Research Article

References

Punja ZK. Biology, ecology and control of Sclerotium rolfsii. Ann. Rev. Phytopathol. 1985;23:97-127.

Sarma BK, Singh DP, Mehta S, Singh HB, Singh UP. Plant growth-promoting rhizobacteria-mediated alterations in phenolic profile of chickpea (Cicer arietinum) infected by Sclerotium rolfsii. J. Phytopathol. 2002;150:277-282.

Kator L, Hosea ZY, Oche OD. Sclerotium rolfsii; Causative organism of southern blight, stem rot, white mold and sclerotia rot disease. Annals of Biological Research. 2015;6(11):78-89.

Weller DM. Biological control of soil borne plant pathogens in the rhizosphere with the bacteria. Ann. Rev. Phytopathol. 1988;26: 261-272.

Thomashow LS, Weller DM. Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici. J. Bacteriol. 1988;170:3499-3508.

Dowling DN, O’Gara F. Metabolites of Pseudomonas involved in the biocontrol of plant disease. Tibtech. 1994;12:133-141.

Anuratha CS, Gnanamanickam SS. Biological control of bacterial wilt caused by Pseudomonas solanacearum in India with antagonistic bacteria. Pl. Soil. 1990;124:109-116.

Yeole RD, Dube HC. Siderophore mediated antibiotics of rhizobacterial fluorescent pseudomonads against soilborne fungal plant pathogens. J. Mycol. Pl. Pathol. 2000;30:335-338.

Sivaprasad P. Microbial inoculant technology for plant disease management. Research Extension Interface, Farm information Bureau, Government of Kerala. 2002;23-30.

Saharan BS, Nehra V. Plant growth promoting rhizobacteria: A critical review. Life Science and Medicine Research. 2011;21:1-30.

Salman M, Abuamsha R, Barghouthi S. Interaction of fluorescent pseudomonads with Pythium ultimum and Rhizoctonia solani in cucumber roots. American Journal of Agricultural Economics. 2013;3:240-251.

Kumar SS, Rao RKM, Kumar RD, Sachin P, Prasad CS. Biocontrol by plant growth promoting rhizobacteria against black scurf and stem canker disease of potato caused by Rhizoctonia solani. Archives of Phytopathology and Plant Protection. 2013;46:487-502.

Beneduzi A, Ambrosini A, Passaglia LMP. Plant growth-promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents. Genetics and Molecular Biology. 2012;35:1044-1051.

Elad Y, Baker R. The role of competition for iron and carbon in suppression of chlamydospore germination of Fusarium oxysporum. Phytopathology. 1985;75:190-195.

Elad Y, Chet I. Possible role of competition for nutrition in biocontrol of Pythium damping-off by bacteria. Phytopathology. 1987;77:190-195.

Pierson LS, Thomashow LS. Cloning and heterologous expression of the phenazine biosynthetic locus from Pseudomonas aureofaciens. Mol. Plant-Microbe Interact. 1992;5:330-339.

Lemanceau P, Bakker PAHM, Dekogel, WJ, Alabouvette C, Schippers B. Effect of pseudobactin 358 produced by Pseudomonas putida WSC358 on suppression of Fusarium wilt of carnations by non pathogenic Fusarium oxysporum. Appl. Environ. Microbiol. 1992;58:2978-2980.

Gull M, Hafeez FY. Characterization of siderophore producing bacterial strain Pseudomonas fluorescens Mst 8.2 as plant growth promoting and biocontrol agent in wheat. African Journal of Microbiology Research. 2012;6:6308-6318.

Frindlender M, Inbar J, Chet I. Biological control of soilborne plant pathogens by a β-1, 3 glucanase producing Pseudomonas cepacia. Soil Biol. Biochem. 1993;25:1211-1221.

Lim H, Kim Y, Kim S. Pseudomonas stutzeri YLP-1 genetic transformation and antifungal mechanism against Fusarium solani, an agent of plant root rot. Appl. Environ. Microbiol. 1991;57:510-516.

Potgieter H, Alexander M. Susceptibility and resistance of several fungi to microbial lysis. J. Bacteriol. 1996;91:1526-1532.

Velazhahan R, Samiyappan R, Vidhyasekaran P. Relationship between antagonistic activities of Pseudomonas fluorescens isolates against Rhizoctonia solani and their production of lytic enzyme. J. Plant Dis. Prot. 1999;106:244-250.

Defago G, Berling CH, Burger U, Hass D, Kahr G, Keel C, Voisard C, Wirthner P, Wuthrich B. Suppression of black root rot of tobacco and other root diseases by strains of Pseudomonas yuorescens: potential applications and mechanisms. In: Hornby D. (Ed.), Biological Control of Soilborne Plant Pathogens. CAB Inter-national, Wellingford, Oxon, UK. 1990;93-108.

Borowitz JJ, Stankie-Dicz M, Lewicka T, Zukowska Z. Inhibition of fungal cellulase, pectinase and xylanase activity of plant growth-promoting fluorescent pseudo-monads. Bull. OILB/SROP. 1992;15:103-106.

Duffy BK, Defago G. Zinc improves biocontrol of Fusarium crown and root rot of tomato by Pseudomonas fuorescens and represses the production of pathogen metabolites inhibitory to bacterial antibiotic biosynthesis. Phyotpathology. 1997;87: 1250-1257.

Garcia-Gutierrez L, Romero D, Zeriouh H, Cazorla FM, Torés JA, Vicente A. Isolation and selection of plant growth-promoting rhizobacteria as inducers of systemic resistance in melon. Plant and Soil; 2012.
DOI: 10.1007/s11104-012-1173-z

Singh UP, Sarma BK, Singh DP. Effect of plant growth-promoting rhizobacteria and culture filtrate of Sclerotium rolfsii on phenolic and salicylic acid contents in chickpea (Cicer arietinum L.). Curr. Microbiol. 2003;46:131-140.

Mari SY, Sundin PB, Waechter-Kristensen J. Induction of phenolic compounds in tomato by rhizosphere bacteria. In: Ogoshi A, Kobayashi K, Homma Y, Kodama F, Kondo N, Akino S, (eds). Plant growth-promoting rhizobacteria-present status and future prospects. Proceedings Fourth Int. Workshop on Plant Growth-Promoting Rhizobacteria Japan-OECD Joint Workshop, Sapporo, Japan. 1997;340-344.

Wei G, Kloepper JW, Tuzun S. Induction to systemic resistance of cucumber to Colletotrichum orbiculare by selected strains of plant growth-promoting rhizobacteria. Phytopathology. 1991;81: 1508-1512.

Sahni S, Prasad BD. Exploitation of pseudomonads for their plant growth-promoting traits. International Journal of Chemical Studies. 2018;SP4:05-10.

Johnson LF, Curl EA. Methods for research on ecology of soil borne plant pathogens. Burgess Publishing Co., Monneapolis. 1972;247.

Ganeshan G, Kumar MA. Pseudomonas fluorescens, a potential bacterial antagonist to control plant diseases. J Plant Interact. 2005;1(3):123-134.

Thomashow LS, Weller DM. Role of antibiotics and siderophores in biocontrol of take all disease of wheat. Plant and Soil. 1990;129:93-99.

Van Elsas JD, Van Overbeek LS, Feldmann AM, Dullemans AM, de Leeuw O. Survival of genetically engineered Pseudomonas fluorescence in soil in competition with the parent strain. FEMS Microbiology Ecology. 1991;85:53-64.

Araujo MAV, Mendoncea-Hagler LC, Hagler AN, Van Elsas JD. Survival of genetically modified Pseudomonas fluorescence introduced into subtropical soils microcosms. FEMS Microbiology Ecology .1994;13:205-216.

Kudryashova EB, Vinokurova NG, Ariskina EV. Bacillus subtilis and phenotypically similar strains producing hexaene antibiotics. Appl. Biochem. Microbiol. 2005; 41(5):486-489.

Antoun H, Prévost D. Ecology of plant growth promoting rhizobacteria. In: Siddiqui ZA. (Ed.), PGPR: Biocontrol and biofertilization, Springer, Dordrecht. 2005; 1–38.

Okamoto H, Sato M, Sato Z, Isaka M. Biocontrol of Phytophthora capsici by Serratia marcescens F-1-1 and analysis of bioc ontrol mechanisms using transposon-insertion mutants. Ann. Phytopathol. Soc. Japan. 1998;64:287-293.

O’Sullivan DJ, O’Gara F. Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens. Microbiol. Rev. 1992;56:662–676.